Rabbit anti-Human CD172b Monoclonal Antibody for Flow Cytometry - O00241

Core Product Specifications and Parameters

Biological Background: CD172b Function and Localization

• Signal-regulatory protein beta-1 (SIRPB1), also known as CD172b, is a cell surface receptor of the immunoglobulin superfamily (IgSF) containing immunoglobulin domains, a transmembrane helix, and a signaling cytoplasmic tail.
• It negatively regulates receptor tyrosine kinase-coupled signaling processes and participates in the recruitment of tyrosine kinase SYK.
• When associated with the adaptor TYROBP, SIRPB1 triggers activation of myeloid cells, playing an immunomodulatory role. Related references: PMID:10604985
• SIRPB1 localizes to the cell membrane and is primarily expressed in monocytes and dendritic cells.
• The protein exists in alternatively spliced isoforms, which may modulate its function; it contains disulfide bonds and is N-glycosylated.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30–371 of human CD172b, corresponding to the extracellular region, which favors recognition of native cell surface receptor in flow cytometry.
• This -conjugated antibody is validated for direct immunofluorescence staining and flow cytometry; consider titrating the optimal concentration for your specific sample.
• Include appropriate controls such as isotype-matched negative control antibody and, if possible, a known positive cell type (e.g., human monocytes) to validate staining specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to validated IVD raw materials, including conjugated monoclonal antibodies like this anti-CD172b clone. We offer a broad portfolio of immune checkpoint and myeloid cell markers, bulk ancillary reagents, and custom rare-target sourcing. Contact us to integrate this reagent into your next diagnostic or research workflow.