Rabbit anti-Human CD172b Monoclonal Antibody for Flow Cytometry - O00241

Core Product Specifications and Parameters

Biological Background: CD172b Function and Localization

• Signal-regulatory protein beta-1 (SIRP-beta-1), also known as CD172b or CD172 antigen-like family member B, is a single-pass type I membrane protein.
• Functions as an immunoglobulin-like cell surface receptor that negatively regulates receptor tyrosine kinase-coupled signaling processes.
• Recruits tyrosine kinase SYK and triggers activation of myeloid cells when associated with TYROBP. Related references: PMID:10604985
• Located on the cell membrane, with an extracellular region containing immunoglobulin-like domains and a cytoplasmic tail for signaling.
• Expressed in monocytes and dendritic cells, suggesting a role in innate immune regulation.
• Contains a signal peptide, multiple immunoglobulin-like domains, a transmembrane helix, and a cytoplasmic domain.
• Undergoes alternative splicing, producing multiple isoforms.
• Post-translational modifications include glycosylation and disulfide bond formation, essential for structural stability.

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , a photostable fluorescent dye suitable for flow cytometry; protect from light and avoid freeze-thaw cycles.
• Since the immunogen encompasses the extracellular domain (aa 30-371), the antibody likely recognizes native surface CD172b; include an appropriate isotype control.
• Titrate the antibody in the range of 0.1–10 µg/mL for flow cytometry to determine the optimal staining concentration.
• Validate performance in known CD172b-expressing cells, such as monocytes and dendritic cells, using positive and negative controls.
• For intracellular applications, assess compatibility with fixation and permeabilization reagents, as epitope accessibility may vary.
• Consider co-staining with myeloid markers to accurately gate CD172b-positive populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw material sourcing, technical services, and expert consulting—supporting every phase from concept to clinical application. For immune checkpoint and myeloid cell research, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, ancillary reagents, and rare target materials like anti-CD172b conjugates for flow cytometry. Streamline your diagnostic development with CamelBio’s reliable supply chain and tailored solutions.