ABflo 647 Rabbit anti-Human CD172a/SIRPα Monoclonal Antibody for Flow Cytometry - P78324

Core Product Specifications and Parameters

Biological Background: CD172a/SIRPα Function and Localization

• SIRPα (signal-regulatory protein alpha), also known as SHPS-1, CD172a, and MYD-1, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily (UniProt P78324).
• It functions as an immunoglobulin-like cell surface receptor for CD47, mediating cell-cell interactions and signaling (By similarity).
• Upon CD47 binding, SIRPα acts as a docking protein, recruiting and translocating tyrosine phosphatases PTPN6 and PTPN11 to the plasma membrane, thereby negatively regulating phagocytosis, mast cell activation, and dendritic cell activation (By similarity).
• SIRPα is involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors, or insulin (By similarity).
• It plays a role in antiviral immunity by limiting new world arenavirus infection through decreased virus internalization (By similarity).
• Acts as a receptor for thrombospondin-1 (THBS1), mediating phosphorylation and ROS production via NADPH oxidase (PubMed:24511121). Related references: PMID:24511121
• Supports adhesion of cerebellar neurons, neurite outgrowth, and glial cell attachment; may play a key role in synaptogenesis and synaptic function (By similarity).
• Ubiquitously expressed, with high levels in brain; detected on myeloid cells and at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle, and pancreas.
• Subcellularly localized to the membrane, featuring extracellular immunoglobulin-like domains, a single transmembrane helix, and a cytoplasmic tail with ITIM motifs; subject to alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 31–370) of human SIRPα, ideally positioning this ABflo 647 conjugate for detection of surface-expressed protein in live, unfixed cells.
• For flow cytometry, consider using human peripheral blood mononuclear cells, monocyte-derived dendritic cells, or SIRPα-positive cell lines; include a CD47-Fc blocking control to verify specific binding.
• As a directly conjugated antibody, no secondary staining step is required—optimize titration in the relevant sample system to balance signal-to-noise ratio.
• Ensure appropriate isotype control and viability dye usage to gate on live, single-cell populations; avoid over-interpretation of expression in mixed cultures.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a reliable single-source supply of validated IVD raw materials, including this anti-SIRPα monoclonal antibody for immune checkpoint and phagocytosis research. Beyond individual reagents, we offer matched antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to accelerate your assay development. Partner with us to streamline procurement from concept to clinic, backed by our integrated technical consulting and supply chain expertise.