Rabbit anti-Human CD172a/SIRPα Monoclonal Antibody for FC - P78324

Core Product Specifications and Parameters

Biological Background: CD172a/SIRPα (Tyrosine-protein phosphatase non-receptor type substrate 1) Function and Localization

• Also known as BIT, MFR, SHPS-1, MYD-1, and p84, CD172a/SIRPα is an immunoglobulin-like cell surface receptor that binds CD47 with high affinity.
• Acts as a docking protein, recruiting the tyrosine phosphatases PTPN6 and PTPN11 to the plasma membrane upon ligand engagement, thereby modulating downstream signaling.
• Functions as an inhibitory receptor, negatively regulating phagocytosis, mast cell activation, and dendritic cell maturation; CD47 binding prevents dendritic cell cytokine production.
• Serves as a receptor for thrombospondin-1 (THBS1), triggering phosphorylation and NADPH oxidase-derived ROS production in non-phagocytic cells. Related references: PMID:24511121
• Plays a role in neuronal adhesion, neurite outgrowth, and synaptic function, supporting cerebellar neuron attachment and glial cell interaction (by similarity).
• Located on the plasma membrane; widely expressed with highest levels in brain, and also detected on myeloid cells but not T-cells.
• Annotated with keywords including glycoprotein, phosphoprotein, disulfide bond, and immunoglobulin domain; it undergoes alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 31-370) spans the extracellular domain of CD172a/SIRPα, making this antibody well-suited for detecting the native surface receptor in flow cytometry (FC) without permeabilization.
• As a rabbit monoclonal antibody, it is expected to exhibit high specificity; nevertheless, optimal staining concentrations and incubation conditions should be titrated for each cell line or primary sample.
• Cross-reactivity beyond human has not been characterized; if used with other species, validate binding beforehand.
• For applications beyond FC, such as immunohistochemistry or Western blotting, note that the epitope may be sensitive to fixation or denaturation, and experimental conditions should be optimized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw material partner, offering diagnostic developers a single access point for antibodies, antigens, and ancillary reagents—from early R&D through clinical application. This CD172a/SIRPα monoclonal antibody for immune checkpoint and phagocytosis research is one example of our expanding catalog; we also support custom sourcing for rare targets and validated antibody pairs. Reach out to explore how CamelBio can simplify your supply chain with integrated procurement and technical consultation.