Rabbit anti-Human CD171/L1CAM Monoclonal Antibody for Flow Cytometry - P32004

Core Product Specifications and Parameters

Biological Background: CD171/L1CAM Function and Localization

• Encoded by the L1CAM gene (also known as CAML1, MIC5, CD171), L1CAM is a neural cell adhesion molecule belonging to the immunoglobulin superfamily, characterized by multiple immunoglobulin domains and fibronectin type-III repeats.
• Plays a pivotal role in cell adhesion dynamics and transmembrane signaling at tyrosine kinase receptors, influencing neuronal development.
• During brain development, L1CAM is critical for neuronal migration, axonal growth, fasciculation, and synaptogenesis.
• In the mature brain, it contributes to the maintenance of neuronal structure and function, including synaptic plasticity.
• Localizes to the cell membrane and is enriched in neuronal projections such as growth cones, axons, and dendrites.
• Undergoes alternative splicing and post-translational modifications including phosphorylation and glycosylation.
• Mutations in L1CAM are associated with neurological disorders, including hereditary spastic paraplegia, Hirschsprung disease, and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 20–1120, covering the majority of the extracellular domain, making this antibody suitable for detecting surface-expressed L1CAM in flow cytometry without permeabilization.
• For optimal FC results, titrate the antibody in the relevant cell system and include appropriate isotype and staining controls.
• Since L1CAM is a transmembrane protein, ensure gentle cell handling to maintain membrane integrity.
• Avoid repeated freeze-thaw cycles of the antibody and protect the fluorophore conjugate from prolonged light exposure.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to high-quality IVD raw materials. For neuroscience and developmental biology researchers focusing on neural adhesion molecules like CD171/L1CAM, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support your assay development from concept to clinic.