Rabbit anti-Human CD171/L1CAM Monoclonal Antibody for Flow Cytometry - P32004

Core Product Specifications and Parameters

Biological Background: Neural Cell Adhesion Molecule L1 Function and Localization

• Neural cell adhesion molecule L1 (L1CAM) is a transmembrane glycoprotein of the immunoglobulin superfamily, involved in cell adhesion and transmembrane signaling at tyrosine kinase receptors.
• During brain development, L1CAM is critical for neuronal migration, axonal growth and fasciculation, and synaptogenesis.
• In the mature brain, it contributes to synaptic plasticity and the maintenance of neuronal structure and function.
• The protein localizes to the cell membrane and is enriched in specialized compartments such as growth cones, axons, and dendrites.
• L1CAM is implicated in several neurological disease variants, including hereditary spastic paraplegia, Hirschsprung disease, intellectual disability, and neurodegeneration.
• Post-translational modifications include N-linked glycosylation, phosphorylation, and disulfide bond formation, contributing to its adhesive and signaling functions.
• The calculated molecular weight of the canonical isoform is 140,003 Da.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fragment spanning amino acids 20–1120 (full-length ectodomain), potentially eliciting antibodies against multiple epitopes; confirm specificity in relevant human cell models.
• This conjugate is suitable for direct flow cytometry; perform titration experiments to determine the optimal antibody concentration and include appropriate isotype and fluorescence-minus-one controls.
• Consider antigen density and receptor internalization dynamics on your target cells; fixation and permeabilization protocols should be optimized if intracellular staining is required.
• Validate performance in relevant sample systems, particularly those expressing L1CAM endogenously (e.g., neuronal cell lines or primary cells), to ensure biologically accurate results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutions with one-stop access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For your neural adhesion and cell surface biomarker projects targeting L1CAM/CD171, we offer consistent, high-quality sourcing of monoclonal antibodies, customized conjugates, and complementary reagents. Whether you need rare-target protein reagents or scalable supply for in vitro diagnostics, CamelBio bridges the gap from concept to clinic.