Rabbit anti-Human CD171/L1CAM mAb - P32004

Core Product Specifications and Parameters

Biological Background: CD171/L1CAM Function and Localization

• L1CAM encodes the neural cell adhesion molecule L1 (also known as NCAM-L1 or CD171), a transmembrane glycoprotein belonging to the immunoglobulin superfamily.
• Functions in cell adhesion dynamics and transmembrane signaling at tyrosine kinase receptors, regulating neuronal migration, axonal growth, fasciculation, and synaptogenesis during brain development.
• In the mature brain, plays a role in synaptic plasticity and maintenance of neuronal structure and function.
• Localizes to the cell membrane, enriched in growth cones, axons, and dendrites of developing and mature neurons.
• Post-translational modifications include phosphorylation, glycosylation, and disulfide bond formation, which are critical for its adhesive and signaling properties.
• Genetic variants in L1CAM are associated with a spectrum of neurological disorders, including hereditary spastic paraplegia, Hirschsprung disease, and X-linked intellectual disability (e.g., MASA syndrome).

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 20–1120), suggesting the antibody likely recognizes an extracellular epitope suitable for flow cytometry on live or lightly fixed cells.
• Titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and confirm reactivity in human samples expressing CD171/L1CAM, such as neuronal cell lines or transfected cells.
• Consider validating the antibody in immunocytochemistry or immunohistochemistry if supported by the supplier’s data, given the protein’s cell surface and neuronal projection localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive IVD raw material access, including validated monoclonal antibodies for neuroscience markers like CD171/L1CAM. We offer pre-optimized antibody pairs, bulk ancillary reagents, and sourcing services for rare or emerging targets to accelerate your assay development from concept to clinic.