Rabbit anti-Human CD170/Siglec-5 mAb for FC - O15389

Core Product Specifications and Parameters

Biological Background: Sialic acid-binding Ig-like lectin 5 Function and Localization

• SIGLEC5 encodes Sialic acid-binding Ig-like lectin 5, also known as CD170, CD33 antigen-like 2 (CD33L2), and obesity-binding protein 2 (OB-BP2).
• It functions as a putative adhesion molecule that mediates sialic-acid dependent binding to cells, recognizing both α-2,3- and α-2,6-linked sialic acids with comparable affinity.
• The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface, modulating ligand binding.
• Siglec-5 is a type I transmembrane protein localized to the cell membrane, featuring an extracellular region with V-set and C2-set immunoglobulin-like domains, a transmembrane segment, and a cytoplasmic tail.
• The protein contains disulfide bonds and is glycosylated, characteristic of immunoglobulin superfamily members.
• Expression is restricted to monocytic/myeloid lineage cells; it is highly expressed in peripheral blood leukocytes, spleen, and bone marrow, with lower levels in lymph node, lung, appendix, placenta, pancreas, and thymus. It is present on monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.
• The UniProt curated entry lists keywords including 3D-structure, cell adhesion, lectin, immunoglobulin domain, glycoprotein, and membrane.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full extracellular region (amino acids 17–434), including all three Ig-like domains, likely preserving native conformational epitopes important for recognition.
• For flow cytometry, titrate the antibody on relevant human myeloid cell lines or primary peripheral blood cells (e.g., monocytes, neutrophils) to determine the optimal concentration.
• Consider sialidase pretreatment of cells to remove cis-interacting sialic acids, which may unmask the epitope and enhance staining intensity in some settings.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to gate for Siglec-5-positive populations accurately.
• Validate antibody performance in the specific sample system of interest, as expression levels may vary with differentiation state and tissue source.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, providing high-quality antibodies, antigen pairs, and ancillary reagents for diagnostic development. This Siglec-5 antibody supports immuno-oncology and myeloid cell characterization workflows. Our platform offers validated monoclonal and polyclonal antibodies, bulk procurement options, and expertise in rare target sourcing to streamline your assay development from concept to clinic.