Rabbit anti-Human CD16b Monoclonal Antibody for Flow Cytometry - O75015

Core Product Specifications and Parameters

Biological Background: Low affinity immunoglobulin gamma Fc region receptor III-B (CD16b) Function and Localization

• CD16b (FCGR3B) is a low affinity receptor for the Fc region of immunoglobulin gamma (IgG) and binds both complexed/aggregated and monomeric IgG. Unlike its paralog CD16a (FCGR3A), it does not mediate antibody-dependent cellular cytotoxicity (ADCC) or phagocytosis.
• It may function as a trap for circulating immune complexes, preventing neutrophil activation while helping clear IgG-antigen aggregates from the peripheral circulation.
• The protein is expressed specifically on polymorphonuclear leukocytes (neutrophils) and also on stimulated eosinophils, distinguishing it from the broader expression pattern of CD16a.
• CD16b is an alternative splicing product of the FCGR3B gene; it is membrane‑anchored via a glycosylphosphatidylinositol (GPI) moiety, lacking a transmembrane domain.
• In addition to its full‑length membrane form, a secreted form can be generated by proteolytic shedding, further extending its immune‑regulatory roles.
• Key structural features include two immunoglobulin‑like domains, conserved disulfide bonds, and multiple N‑glycosylation sites critical for ligand binding and stability.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (amino acids 20–208), targeting the ligand‑binding region; it is expected to recognize both membrane‑bound and soluble forms of CD16b.
• This antibody is directly conjugated to , a photostable far‑red fluorophore, and is suitable for flow cytometry without the need for a secondary staining step.
• For optimal cell‑surface staining, use washed whole blood or purified neutrophils; fixation/permeabilization is not required unless intracellular or total protein detection is desired.
• Because CD16b expression is restricted to neutrophils and stimulated eosinophils, always include proper isotype controls and a pan‑leukocyte marker (e.g., CD45) to validate cell‑type specificity.
• When analyzing immune complex binding, consider pre‑blocking with human serum or purified IgG to minimize non‑specific interactions via Fc receptors other than CD16b.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for IVD raw‑material procurement, offering validated monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized development services. This anti‑CD16b conjugate supports immunology and hematology research flow cytometry panels, and our one‑stop platform can also source rare‑target antibodies and matched antibody pairs to streamline your assay development from concept to clinic.