Rabbit anti-Human CD16b mAb for FC - O75015

Core Product Specifications and Parameters

Biological Background: Low affinity immunoglobulin gamma Fc region receptor III-B Function and Localization

• Protein identity: CD16b (FCGR3B) is the low-affinity IgG Fc receptor III-B, a GPI-anchored glycoprotein belonging to the immunoglobulin superfamily. It is also known as CD16b, FcRIIIb, or FcR-10.
• Gene family: FCGR3B is a member of the Fc gamma receptor gene family, encoding receptors for the Fc domain of IgG antibodies, which play critical roles in immune responses.
• Core function: Functions as a low-affinity receptor for the Fc region of IgG. It binds complexed or aggregated IgG and monomeric IgG, but unlike CD16a (FCGR3A), it cannot mediate antibody-dependent cellular cytotoxicity or phagocytosis. It may act as a trap for immune complexes in circulation without activating neutrophils.
• Subcellular localization: Expressed on the cell membrane of neutrophils via a GPI anchor; also found in a secreted form.
• Tissue specificity: Specifically expressed by polymorphonuclear leukocytes (neutrophils) and stimulated eosinophils.
• Keywords and PTMs: Contains an immunoglobulin domain, a signal peptide, disulfide bonds, N-linked glycosylation sites, and a GPI-anchor attachment site. It lacks the transmembrane and cytoplasmic domains found in the closely related FCGR3A.
• Structural note: The protein has a molecular weight of approximately 26 kDa (calculated), consistent with its extracellular Ig-like domains and GPI modification.

Experimental Guidance and Technical Tips

• This antibody was generated against a recombinant immunogen spanning the extracellular region (aa 20-208) and is likely to recognize the native conformation of CD16b on live cells, making it suitable for flow cytometry.
• For FC staining, titrate the antibody to determine the optimal concentration for your specific cell system and consider using appropriate isotype controls.
• CD16b is selective for neutrophils; ensure proper gating strategies and include neutrophil markers to accurately identify target populations.
• As an conjugate, it provides bright and photostable fluorescence, compatible with common laser lines (e.g., 488 nm) and multicolor panels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering validated antibodies, antigens, and ancillary reagents from concept to clinic. This anti-CD16b monoclonal antibody supports neutrophil biology and immune complex research in flow cytometry applications. For bulk quantities, custom conjugation, or access to a wide portfolio of rare-target antibodies, contact CamelBio for reliable sourcing and technical support.