Rabbit anti-Human CD166/ALCAM Monoclonal Antibody for Flow Cytometry - Q13740

Core Product Specifications and Parameters

Biological Background: CD166/ALCAM Function and Localization

• CD166/ALCAM, also known as activated leukocyte cell adhesion molecule, is a cell adhesion molecule that mediates both heterotypic (with CD6) and homotypic cell-cell contacts, playing a key role in T-cell activation and immunological synapse formation. Related references: PMID:15048703, PMID:15496415, PMID:16352806, PMID:24945728, PMID:15294938
• It acts as a ligand for the inhibitory receptor LILRB4, suppressing T cell proliferation, and is essential for hematopoietic stem cell engraftment in the bone marrow. Related references: PMID:29263213, PMID:24740813
• ALCAM homotypic interactions facilitate dendritic cell attachment to endothelial cells and inhibit endothelial cell migration while promoting tube formation. Related references: PMID:23169771, PMID:15496415
• An alternatively spliced secreted isoform antagonizes membrane-bound ALCAM functions, inhibiting cell attachment and promoting endothelial cell migration.
• Subcellularly, ALCAM is localized to the cell membrane, including axon and dendrite projections, and can be secreted.
• Tissue expression is broad: detected on hematopoietic stem cells, activated T/B cells, monocytes, dendritic cells, and various endothelial and epithelial cells; elevated in metastatic melanoma lines. Related references: PMID:24740813, PMID:23169771, PMID:15048703, PMID:16352806, PMID:9502422, PMID:7760007, PMID:15496415
• The protein contains immunoglobulin-like domains, is glycosylated, and has disulfide bonds; the canonical isoform has a predicted molecular weight of ~65 kDa, but multiple bands may be observed due to splicing and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (aa 28–526), which includes the ligand-binding region; this suggests the antibody likely recognizes an epitope accessible on live cells, suitable for flow cytometry.
• For flow cytometry, titrate the antibody to determine the optimal staining concentration for your cell type; include appropriate isotype controls.
• Because ALCAM expression varies among cell types and activation states, validate staining on known positive (e.g., activated T cells, dendritic cells) and negative controls.
• Multiple protein bands (15–65 kDa) annotated in the specifications may reflect splice variants or cleavage products; if performing western blotting, confirm specificity with a lysate from ALCAM-overexpressing cells.
• This antibody is validated for human reactivity; cross-reactivity with other species is not reported and should be tested if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with streamlined access to premium IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. This CD166/ALCAM antibody serves immunology, oncology, and stem cell research sectors where robust cell adhesion and activation markers are critical. Whatever your sourcing needs—from rare targets to bulk supply—CamelBio provides tailored procurement support to accelerate your development pipeline.