Rabbit anti-Human CD166/ALCAM Monoclonal Antibody for FC - Q13740

Core Product Specifications and Parameters

Biological Background: CD166/ALCAM Function and Localization

• Mediates heterotypic cell-cell contacts through interaction with CD6, promoting T-cell activation and proliferation. Related references: PMID:15048703, PMID:16352806, PMID:24945728
• Mediates homotypic cell-cell contacts, contributing to immunological synapse formation and T-cell activation. Related references: PMID:15496415, PMID:16352806
• Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation. Related references: PMID:29263213
• Required for normal hematopoietic stem cell engraftment in the bone marrow and for maintaining hematopoietic stem cell numbers. Related references: PMID:24740813
• Mediates attachment of dendritic cells onto endothelial cells, inhibits endothelial cell migration, and promotes endothelial tube formation via homotypic interactions. Related references: PMID:15496415, PMID:23169771
• Promotes neurite extension, axon growth, and axon guidance; involved in retinal ganglion cell axon outgrowth and pathfinding (By similarity).
• Subcellular localization includes cell membrane, cell projections (axon, dendrite), and a secreted form.
• Expressed on activated T-cells, B-cells, monocytes, hematopoietic stem cells, endothelial cells, neurons, and highly metastasizing melanoma cell lines.

Experimental Guidance and Technical Tips

• Titrate the antibody to determine the optimal dilution for flow cytometry, as staining intensity varies with antigen density.
• Use appropriate isotype control antibodies and live/dead exclusion dyes to ensure accurate gating.
• Validate performance in relevant cell systems (e.g., activated PBMCs, Jurkat cells, or A375 melanoma line) expressing CD166/ALCAM.
• requires excitation ~590 nm and emission ~617 nm; ensure instrument configuration matches these spectral properties.
• Protect antibody from prolonged light exposure to maintain conjugate stability.
• Store undiluted at 2–8°C; avoid repeated freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio accelerates IVD development by offering fully optimized monoclonal antibodies, including fluorophore-conjugated formats for direct flow cytometry applications. Our CD166/ALCAM product exemplifies our commitment to rare-target sourcing, backed by bulk ancillary reagents and validated antibody pairs to support your diagnostic pipeline from concept to clinic.