Rabbit anti-Human CD162/PSGL-1 Monoclonal Antibody for Flow Cytometry - Q14242

Core Product Specifications and Parameters

Biological Background: CD162/PSGL-1 Function and Localization

• PSGL-1 (P-selectin glycoprotein ligand 1), also known as CD162 or selectin P ligand, is a SLe(x)-type proteoglycan.
• It mediates leukocyte rolling on vascular surfaces through high-affinity, calcium-dependent interactions with E-, P-, and L-selectins, a critical initial step in inflammation.
• PSGL-1 is expressed primarily on neutrophils, monocytes, and most lymphocytes.
• The protein functions as a receptor for enterovirus 71, facilitating host cell entry during infection.
• PSGL-1 is a transmembrane protein located at the cell membrane, with a single-pass transmembrane domain.
• Post-translational modifications include sulfation, sialic acid attachment, and cleavage on dibasic residues, which are essential for selectin binding.
• Alternative splicing generates multiple isoforms; the canonical isoform has a calculated molecular mass of ~43 kDa, matching the observed 43-44 kDa range.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 42-295), covering the region responsible for selectin binding and virus interaction. This design likely detects all known splice variants.
• This conjugate is recommended for direct flow cytometry on live human leukocytes. Validate staining with appropriate controls (e.g., unstimulated vs. activated cells or isotype controls) in your specific experimental setup.
• PSGL-1 is heavily glycosylated; denaturing conditions in western blotting may not preserve the epitope, so confirm suitability if switching applications.
• Protect the conjugate from light and avoid multiple freeze-thaw cycles to maintain fluorescence intensity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For immunology and inflammation research, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Whether you're developing flow cytometry assays or investigating leukocyte adhesion pathways, CamelBio connects you with the critical reagents to advance your projects efficiently.