Rabbit anti-Human CD162/PSGL-1 mAb for FC - Q14242

Core Product Specifications and Parameters

Biological Background: CD162/PSGL-1 Function and Localization

• P-selectin glycoprotein ligand 1 (PSGL-1), also known as CD162 or selectin P ligand, is encoded by the SELPLG gene. It is a SLe(x)-type proteoglycan that functions as a key adhesion molecule in the immune system.
• PSGL-1 mediates calcium-dependent interactions with E-, P-, and L-selectins, enabling the tethering and rolling of leukocytes on endothelial cells, which is the initial step in leukocyte extravasation during inflammation.
• It plays a critical role in the early leukocyte capture and is essential for directing leukocytes to sites of inflammation or injury.
• Besides its role in inflammation, PSGL-1 serves as a receptor for enterovirus 71, highlighting its involvement in host-pathogen interactions.
• Subcellularly, PSGL-1 is a transmembrane protein located on the cell surface membrane.
• It is expressed predominantly on hematopoietic cells, including neutrophils, monocytes, and most lymphocytes.
• Post-translational modifications include tyrosine sulfation, O-linked sialylation, and glycosylation, which are critical for its selectin-binding activity. The protein also undergoes alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• Since this antibody is directly conjugated to and raised against the extracellular domain (aa 42-295), it is suitable for live-cell flow cytometry staining without a secondary antibody.
• Titrate the antibody to determine the optimal concentration for FC; use an isotype-matched control to assess non-specific binding.
• Validate performance in known PSGL-1-expressing cells such as human neutrophils, monocytes, or lymphocyte subsets.
• Consider using this antibody in combination with other surface markers for detailed immunophenotyping of leukocyte populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and life science companies with one-stop access to high-quality IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Whether you are developing assays for inflammation, leukocyte trafficking, or immune profiling, CamelBio supports your workflow from concept to clinic with reliable material supply. Explore our anti-CD162/PSGL-1 antibody and other selective tools for your next IVD development project.