Rabbit anti-Human CD161 Monoclonal Antibody for Flow Cytometry - Q12918

Core Product Specifications and Parameters

Biological Background: CD161 Function and Localization

• CD161, also known as Killer cell lectin-like receptor subfamily B member 1 (KLRB1) or NKR-P1A, is a type II transmembrane glycoprotein belonging to the C-type lectin family.
• It functions as an inhibitory receptor on natural killer (NK) cells, suppressing cytotoxicity upon ligand engagement.
• Ligand binding activates acid sphingomyelinase/SMPD1, leading to elevated intracellular ceramide levels and downstream signaling via AKT1/PKB and RPS6KA1/RSK1 kinases.
• CD161 also acts as a lectin, binding to terminal Gal-alpha(1,3)Gal and N-acetyllactosamine epitopes, and recognizes CLEC2D/LLT1 as a functional ligand to inhibit NK cell cytotoxicity and interferon-gamma secretion.
• It is expressed predominantly on a subset of NK cells in the intestinal epithelium and liver, as well as on peripheral blood T-cells with a memory phenotype.
• The receptor is anchored in the membrane via a signal-anchor sequence and contains a transmembrane helix, with its extracellular C-type lectin domain stabilized by disulfide bonds and glycosylation.
• Structurally, the mature protein has a predicted molecular weight of approximately 25 kDa, consistent with its glycosylated form.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular lectin domain (aa 67-225), suggesting the antibody binds an epitope accessible on live cells; thus, it is suitable for surface staining in flow cytometry without permeabilization.
• Optimal antibody titration and staining conditions should be determined empirically for each experimental system.
• Since CD161 is expressed on NK cells and memory T-cells, appropriate cell models include peripheral blood mononuclear cells (PBMCs), isolated NK cells, or T-cell lines.
• As an inhibitory receptor, CD161 may be modulated upon cellular activation; researchers should consider activation status when designing experiments.
• Avoid prolonged light exposure to maintain fluorophore stability.
• Use appropriate isotype controls for accurate gating in flow cytometry.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials for immune cell research, including this anti-CD161 antibody. As a one-stop sourcing partner, we supply optimized antibody pairs, monoclonal antibodies, and ancillary reagents to support the development of diagnostic assays targeting NK cell and T-cell biology. Contact us for bulk orders, customized formulations, or assistance with rare target reagent sourcing.