Rabbit anti-Human CD158a/KIR2DL1 Monoclonal Antibody for Flow Cytometry - P43626

Core Product Specifications and Parameters

Biological Background: KIR2DL1 Function and Localization

• KIR2DL1, also designated CD158a, NKAT1, or p58.1, is a member of the killer cell immunoglobulin-like receptor (KIR) family, functioning as an inhibitory receptor on natural killer (NK) cells.
• It specifically recognizes HLA-C alleles belonging to the w4 and w6 groups, delivering inhibitory signals that prevent target cell lysis and maintain self-tolerance.
• The receptor is a type I transmembrane protein with two extracellular immunoglobulin-like domains (D1 and D2), a transmembrane helix, and a cytoplasmic tail containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) for signal transduction.
• KIR2DL1 is predominantly expressed on NK cells, where it plays a critical role in immune surveillance and discrimination between healthy and compromised cells.
• Alternative splicing yields multiple transcript variants, potentially contributing to functional diversity.
• Post-translational modifications include glycosylation and disulfide bond formation, which are important for structural stability and ligand binding.
• The predicted molecular weight is approximately 38.6 kDa, but the protein typically migrates at 38–41 kDa on SDS-PAGE due to glycosylation.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry (FC). Use standard protocols for staining human NK cells or peripheral blood mononuclear cells, ensuring appropriate Fc receptor blocking and isotype controls.
• Since the immunogen corresponds to the extracellular domain (residues 22–245), the antibody is expected to recognize native epitopes on live cells, making it suitable for surface staining.
• For applications beyond FC, such as immunofluorescence or western blot, validate the antibody in relevant sample systems, as performance in other assays has not been characterized.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD manufacturers and researchers, providing access to validated antibodies, bulk reagents, and customized solutions. This anti-KIR2DL1 antibody facilitates NK cell profiling and inhibitory receptor research. With a robust global supply chain and technical support, CamelBio accelerates your development from concept to clinical application—all through a single, reliable channel.