Rabbit anti-Human CD158 (KIR2DL1/S1/S2/S3/S5) Monoclonal Antibody for Flow Cytometry - Q14954 / Q14953 / Q14952 / P43631 / P43626

Core Product Specifications and Parameters

Biological Background: CD158 (KIR2DL1/S1/S2/S3/S5) Function and Localization

• KIR2DS1 (CD158H) is a member of the killer cell immunoglobulin-like receptor (KIR) family, characterized by two extracellular immunoglobulin domains and a short cytoplasmic tail lacking immunoreceptor tyrosine-based inhibitory motifs (ITIMs).
• It functions as an activating receptor on natural killer (NK) cells, specifically recognizing certain HLA-C alleles such as w6, and does not inhibit NK cell activity, promoting cytotoxicity and cytokine release upon ligand binding.
• The KIR gene family is highly polymorphic and is located on chromosome 19q13.4; this antibody cross-reacts with multiple KIR members including KIR2DS1, KIR2DS2, KIR2DS3, KIR2DL1, and KIR2DL3 due to shared epitopes, enabling broad detection of CD158-positive NK cell subsets.
• KIR2DS1 is localized at the cell membrane, typical of type I transmembrane receptors, and is expressed primarily on NK cells, playing a key role in immune surveillance and response to virally infected or transformed cells.
• Post-translational modifications include disulfide bond formation, N-linked glycosylation, and the presence of an N-terminal signal peptide, all contributing to proper folding and surface expression.
• UniProt keywords highlight its characteristics as a membrane receptor, glycoprotein, and member of the immunoglobulin superfamily, with structural repeats common to KIR molecules.

Experimental Guidance and Technical Tips

• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type, typically starting with a range of 0.5–5 µg per test, and include appropriate isotype controls.
• When using in multi-color panels, ensure proper compensation controls are prepared, as the fluorophore may require compensation for spectral overlap with similar dyes.
• Validate the antibody in the relevant sample system, such as human peripheral blood mononuclear cells (PBMCs) or isolated NK cells, and consider using Fc receptor blocking reagents to minimize non-specific binding.
• The immunogen corresponds to amino acids 22-245, which covers the extracellular domain of CD158; thus, the antibody is expected to detect surface-expressed receptors and may not recognize intracellular or unprocessed forms.
• Avoid sodium azide if performing functional assays, as it may interfere with cell viability and NK cell activation; use preservative-free formulations or wash cells thoroughly before downstream applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-CD158 (KIR) antibody supports NK cell receptor analysis and immuno-oncology research, and we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist with rare target raw-material sourcing. Contact us to streamline your IVD development from concept to clinic.