Rabbit anti-Human CD157/BST-1 mAb for Flow Cytometry - Q10588

Core Product Specifications and Parameters

Biological Background: CD157/BST-1 Function and Localization

• CD157/BST-1, encoded by the BST1 gene, is also known as ADP-ribosyl cyclase 2 and cyclic ADP-ribose hydrolase 2.
• It catalyzes both the synthesis of cyclic ADP-β-D-ribose (cADPR) from NAD+ and its hydrolysis to ADP-D-ribose (ADPR). Related references: PMID:7805847
• cADPR serves as an endogenous second messenger that triggers calcium release from intracellular stores, thereby regulating intracellular calcium mobilization.
• CD157/BST-1 may be involved in pre-B-cell growth.
• The protein is anchored to the cell membrane via a GPI anchor.
• It is expressed in various tissues, including placenta, lung, liver, and kidney.
• Post-translational modifications include glycosylation and disulfide bond formation; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• This antibody is validated for flow cytometry; titrate to determine the optimal working dilution for your specific sample and experiment.
• Include appropriate controls such as isotype control antibodies and positive cell lines (e.g., known CD157/BST-1-expressing cells) to confirm specificity.
• Because CD157/BST-1 is a GPI-anchored surface protein, use standard cell surface staining protocols without permeabilization unless intracellular epitopes need to be accessed.
• The conjugate requires appropriate laser excitation (e.g., 561 nm or 594 nm) and emission filter sets — verify compatibility with your instrument.
• As a monoclonal antibody, it recognizes a single epitope; if epitope masking is suspected (e.g., in multicolor panels), empirical validation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CD157/BST-1 antibody belongs to our extensive catalog for immunology and cell biology research. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare target raw materials to accelerate your IVD development.