Rabbit anti-Human CD150/SLAM Monoclonal Antibody for Flow Cytometry - Q13291

Core Product Specifications and Parameters

Biological Background: CD150/SLAM Function and Localization

• SLAMF1 (CD150/SLAM) is a member of the SLAM family of immunoglobulin receptors that acts as a self-ligand, modulating the activation and differentiation of various immune cells (T-cells, B-cells, dendritic cells, macrophages) and interconnecting innate and adaptive immunity.
• In T-cells, SLAMF1 signaling proceeds via two pathways: one dependent on the adaptor SH2D1A/SAP (which recruits FYN kinase) and another mediated by PTPN11 (SHP-2). Related references: PMID:11806999, PMID:12458214
• In B-cells, SLAMF1 activates ERK signaling (SYK- and INPP5D-dependent) as well as Akt, p38 MAPK, and JNK1/2 cascades. Related references: PMID:20231852
• SLAMF1 also functions as a host cell receptor for Measles virus entry, including its isoform 4.
• SLAMF1 is a membrane glycoprotein with disulfide bonds and phosphorylation sites; it localizes primarily at the cell membrane and can be secreted. It is constitutively expressed on memory T-cells and rapidly induced on naive T-cells, with alternative isoforms in B-cells, dendritic cells, and tumors of the central nervous system. Related references: PMID:7617038, PMID:9091591, PMID:25710480
• In the innate immune response, SLAMF1 recognizes Gram-negative bacterial surface proteins (OmpC/OmpF) and regulates phagosome maturation in macrophages. Related references: PMID:20818396
• SLAMF1 inhibits CD40-induced signal transduction in monocyte-derived dendritic cells. Related references: PMID:16317102

Experimental Guidance and Technical Tips

• The antibody is directly conjugated to , a bright red fluorescent dye, making it suitable for direct flow cytometry without a secondary antibody. Titrate to determine the optimal working concentration for your assay.
• Use appropriate Fc receptor blocking (e.g., with human IgG or serum) when staining whole blood or PBMCs to reduce non-specific binding.
• For intracellular detection of CD150, fix and permeabilize cells; note that surface staining may suffice for most immune cell applications.
• Validation in your specific sample type (e.g., activated T-cells, B-cell lines, dendritic cells) is recommended, as expression levels can vary upon activation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic developers from concept to clinic. This anti-CD150 antibody is designed for immunology and infectious disease research, and CamelBio can supply validated antibody pairs, conjugated monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Whatever your diagnostic needs, CamelBio streamlines procurement to keep your development on track.