Rabbit anti-Human CD150/SLAM Monoclonal Antibody for FC - Q13291

Core Product Specifications and Parameters

Biological Background: CD150/SLAM Function and Localization

• Signaling lymphocytic activation molecule (SLAM, also known as CD150 or CDw150) is a self-ligand receptor of the SLAM family, encoded by the SLAMF1 gene. It exists in multiple isoforms, including a membrane-bound form and a secreted variant.
• SLAM modulates the activation and differentiation of a wide variety of immune cells, bridging innate and adaptive immunity. Its signaling is differentially regulated by adaptors SH2D1A/SAP or SH2D1B/EAT-2, leading to distinct outcomes in T and B lymphocytes. Related references: PMID:11806999 PMID:12458214
• In T cells, SLAM promotes IL-2-independent proliferation and IFN-gamma production. It enhances TCR-induced IL-4 secretion by CD4+ cells and supports T follicular helper cell function in germinal centers.
• In B cells, SLAM activates the ERK and Akt pathways via SYK and INPP5D, and induces p38 MAPK, JNK1, and JNK2 signaling, contributing to humoral immune response regulation. Related references: PMID:20231852
• The protein is primarily located at the cell membrane but can also be secreted. It contains one immunoglobulin domain and undergoes phosphorylation, glycosylation, and disulfide bond formation.
• Constitutively expressed on peripheral blood memory T cells, immature thymocytes, and a subset of B cells, SLAM is rapidly upregulated upon naive T-cell activation. Isoform 4 is found in dendritic cells, macrophages, and certain tumors. Related references: PMID:7617038 PMID:9091591 PMID:25710480
• SLAM serves as a receptor for measles virus and plays a role in innate responses to gram-negative bacteria by recognizing bacterial surface proteins OmpC/OmpF, triggering phagosome maturation and NOX2 activity. Related references: PMID:20818396

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 21-237), making the antibody suitable for detecting surface SLAM on live unfixed cells.
• For flow cytometry, titrate the antibody and include appropriate isotype controls to establish optimal staining concentrations for your specific cell system.
• Validate the antibody on known SLAM-positive and -negative cell lines or primary immune cells under activation conditions (e.g., stimulated PBMCs).
• Ensure that your flow cytometer is equipped with a violet laser (405 nm) for detection; compensation may be required in multicolor panels.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw‑material and technical services partner for diagnostic manufacturers, laboratories, and research institutes. This monoclonal antibody targets human CD150/SLAM, a key immune receptor, supporting flow cytometry applications in immunological and infectious disease research. Beyond antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and sourcing for rare‑target raw materials to streamline your assay development from concept to clinic.