Rabbit anti-Human CD150/SLAM Monoclonal Antibody for FC - Q13291

Core Product Specifications and Parameters

Biological Background: CD150/SLAM Function and Localization

• CD150/SLAM (signaling lymphocytic activation molecule) is a self-ligand receptor of the SLAM family that modulates activation and differentiation of a wide variety of immune cells, bridging innate and adaptive immune responses.
• SLAMF1 signaling involves adapter proteins SH2D1A/SAP and SH2D1B/EAT-2, with two modes: one SH2D1A-dependent and another PTPN11-dependent; SH2D1A can simultaneously prevent binding to inhibitory effectors and recruit FYN for phosphorylation. Related references: PMID:11806999 PMID:12458214
• In T-cells, SLAMF1 promotes TCR-induced IL-4 secretion, IL-2-independent proliferation, and IFN-gamma production, while in B-cells it activates ERK, Akt, p38 MAPK, and JNK pathways. Related references: PMID:20231852
• SLAMF1 acts as a receptor for Measles virus, including isoform 4.
• Subcellularly, it is found primarily at the cell membrane, with secreted and membrane-associated forms reported.
• Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes, and a proportion of B-cells; rapidly induced on naive T-cells after activation. Activated B-cells express isoforms 1, 3, and a cytoplasmic isoform. Isoform 4 is expressed in B-cells, primary T-cells, dendritic cells, macrophages, and tumors of the central nervous system. Related references: PMID:7617038 PMID:9091591 PMID:25710480
• SLAMF1 participates in innate immune defense against Gram-negative bacteria in macrophages, likely recognizing OmpC/OmpF, regulating phagosome maturation, and recruiting the PI3KC3-C2 complex. Related references: PMID:20818396
• Post-translational modifications include glycosylation, disulfide bond formation, and phosphorylation; multiple isoforms arise from alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (aa 21–237), making this antibody potentially suitable for detecting surface-expressed CD150 by flow cytometry.
• Consider titrating the antibody to determine the optimal staining concentration for your specific cell type and experimental conditions.
• SLAM expression is upregulated upon lymphocyte activation; ensure appropriate activation conditions if using resting cells.
• For multicolor flow cytometry panels, confirm compensation settings for the fluorophore to minimize spectral overlap.
• Include appropriate isotype controls and fluorescence minus one (FMO) controls to accurately gate CD150-positive populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a single access point for IVD raw materials, technical services, and consulting—from concept to clinic. Our extensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing for immunology and infectious disease research. For CD150/SLAM-focused studies, we can support your project with high-quality reagents and tailored supply solutions.