Rabbit anti-Human CD144/VE Cadherin Monoclonal Antibody for FC - P33151

Core Product Specifications and Parameters

Biological Background: VE-Cadherin Function and Localization

• Cadherin-5 (VE-cadherin) is a classical cadherin that mediates calcium-dependent homophilic cell adhesion, contributing to the sorting of heterogeneous cell types [PMID:21269602]. Related references: PMID:21269602
• In endothelial cells, it plays a critical role in controlling the cohesion and organization of intercellular junctions, associating with alpha-catenin to link adherens junctions to the actin cytoskeleton [PMID:10861224]. Related references: PMID:10861224
• Through interactions with KRIT1 and PALS1, it establishes and maintains endothelial cell polarity and vascular lumen formation by activating the Par polarity complex and RAP1B [PMID:20332120]. Related references: PMID:20332120
• It positively regulates reorientation of actin stress fibers and endothelial cell reorientation in response to cellular mechanotransduction [PMID:25795300]. Related references: PMID:25795300
• VE-cadherin associates with p120-catenin (CTNND1) to regulate cadherin endocytosis and junctional turnover (By similarity).
• Expression is endothelial cell-specific; it is detected at the protein level in endothelial cells and in the brain [PMID:27338829, PMID:2059658]. Related references: PMID:27338829, PMID:2059658
• Undergoes post-translational modifications including proteolytic cleavage and phosphorylation, which regulate its adhesive function and signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 48–593) of human VE-cadherin; consider that the antibody may recognize both full-length and shed ectodomain fragments.
• This antibody is designed for flow cytometry (FC); optimize titration and include appropriate isotype controls and viability dyes to reduce false positives.
• For intracellular staining, permeabilization conditions should be validated, as VE-cadherin is predominantly membrane-localized.
• Confirm expression in your target cell type, as VE-cadherin is endothelial-specific; negative controls such as VE-cadherin-negative cell lines are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive IVD raw-material sourcing partner, providing validated monoclonal antibodies, antibody pairs, and ancillary reagents to accelerate your diagnostic development. Our portfolio, including this anti-CD144/VE-Cadherin antibody, supports endothelial biology and vascular research, helping you transition from concept to clinic. With bulk supply capabilities and access to rare targets, we ensure seamless procurement for your diagnostic pipeline.