Rabbit anti-Human CD144/VE Cadherin mAb for FC - P33151

Core Product Specifications and Parameters

Biological Background: Cadherin-5 Function and Localization

• Cadherin-5 (VE-cadherin, CD144, 7B4 antigen) is a classical cadherin that mediates calcium-dependent homophilic cell-cell adhesion, crucial for endothelial cell sorting and barrier integrity (PubMed:21269602). Related references: PMID:21269602
• It anchors the endothelial adherens junction complex by linking the actin cytoskeleton via alpha-catenin, thereby controlling intercellular cohesion and organization (PubMed:10861224). Related references: PMID:10861224
• In concert with KRIT1 and PALS1, Cadherin-5 orchestrates endothelial cell polarity and vascular lumen formation through recruitment of the Par polarity complex and RAP1B (PubMed:20332120). Related references: PMID:20332120
• It transduces mechanical signals by positively regulating actin stress fiber reorientation and endothelial cell alignment in response to mechanotransduction (PubMed:25795300). Related references: PMID:25795300
• Endocytosis of Cadherin-5 is regulated through association with CTNND1/p120-catenin, modulating junctional dynamics (By similarity).
• Expressed predominantly in endothelial cells and also detected in brain tissue (PubMed:27338829, PubMed:2059658). Related references: PMID:27338829 PMID:2059658
• Subcellularly localized at cell junctions (adherens junctions), cell membrane, and cytoplasm, reflecting its dynamic trafficking and recycling.
• Post-translational modifications include phosphorylation and glycosylation; the protein features a signal peptide, repetitive cadherin domains, and a transmembrane helix, consistent with keywords "Phosphoprotein", "Glycoprotein", and "Signal".

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning the extracellular domain (aa 48-593), suggesting the antibody likely recognizes native conformational epitopes, making it suitable for non-denaturing applications like flow cytometry.
• For flow cytometry, validate antibody concentration and staining conditions using known positive (e.g., HUVEC endothelial cells) and negative controls.
• Since the antibody is only validated for FC, users should perform rigorous optimization and validation if employing it in other applications such as Western blotting, IHC, or ELISA.
• Consider co-staining with endothelial markers (e.g., CD31) to confirm cell type-specific expression in mixed populations.

CamelBio: Your One-Stop Sourcing Bridge

Discover CamelBio's comprehensive portfolio of IVD raw materials, technical services, and consulting, designed to accelerate your diagnostic development from concept to clinic. This anti-CD144/VE-cadherin antibody supports vascular and endothelial cell research, with applications in evaluating cell adhesion, barrier function, and angiogenesis. Leverage our validated antibody pairs, bulk ancillary reagents, and rare target sourcing capabilities to streamline your assay development and manufacturing.