Rabbit anti-Human CD142 (Tissue Factor/TF) Monoclonal Antibody for Flow Cytometry - P13726

Core Product Specifications and Parameters

Biological Background: Tissue Factor Function and Localization

• Tissue factor (TF, CD142) is a transmembrane glycoprotein encoded by the F3 gene, also known as coagulation factor III or thromboplastin.
• It functions as the high-affinity receptor and cofactor for coagulation factor VII/VIIa, initiating the extrinsic blood coagulation cascade.
• The TF:VIIa complex proteolytically activates factor IX and factor X, leading to thrombin generation and fibrin clot formation.
• TF is primarily expressed in tissues surrounding blood vessels, such as lung, placenta, and pancreas, where it provides a hemostatic barrier.
• The protein exists in membrane-bound and secreted forms due to alternative splicing, and undergoes post-translational modifications including disulfide bond formation, glycosylation, and palmitoylation.
• Subcellularly, TF is localized to the plasma membrane, with a single transmembrane helix anchoring it to the cell surface.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , making it ready for flow cytometric detection of surface CD142 expression on human cells without the need for a secondary antibody.
• Perform antibody titration to determine the optimal staining concentration for your specific cell type and experimental conditions.
• Since tissue factor is expressed on the cell surface, live-cell staining without permeabilization is appropriate; consider including a viability dye to exclude dead cells.
• For optimal results, validate the antibody in a relevant biological system known to express tissue factor, such as stimulated human monocytes or endothelial cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to validated IVD raw materials, including this -conjugated anti-CD142 antibody for coagulation and hemostasis studies. Our comprehensive portfolio spans antibodies, antigens, and ancillary reagents, supporting assay development from concept to clinic. Contact us for bulk sourcing, technical consultation, and rare-target material procurement.