Rabbit anti-Human CD142 Monoclonal Antibody for Flow Cytometry - P13726

Core Product Specifications and Parameters

Biological Background: Tissue Factor Function and Localization

• Tissue factor (TF), also known as coagulation factor III, thromboplastin, or CD142, is a transmembrane glycoprotein encoded by the F3 gene.
• TF initiates blood coagulation by forming a complex with circulating factor VII or VIIa; the TF:VIIa complex activates factors IX or X through limited proteolysis, triggering the coagulation cascade.
• It plays a critical role in normal hemostasis by driving cell-surface assembly and propagation of the protease cascade that leads to thrombin formation and clot formation.
• Subcellularly, TF is primarily located at the cell membrane but can also be secreted in a soluble form, as indicated by alternative splicing.
• TF is highly expressed in tissues such as lung, placenta, and pancreas, consistent with its role in rapid hemostatic responses at these sites.
• The protein undergoes post-translational modifications including disulfide bond formation, glycosylation, and palmitoylation, and it contains a transmembrane helix; it is also classified as a lipoprotein.

Experimental Guidance and Technical Tips

• This antibody is directly conjugated to , eliminating the need for secondary detection reagents and reducing non-specific background.
• The immunogen (aa 34-251) corresponds to the extracellular domain, suggesting the antibody recognizes the native, membrane-bound TF; it is validated for flow cytometry.
• For flow cytometry, titrate the antibody on a human cell line known to express CD142 (e.g., THP-1, HUVEC) and include appropriate isotype and viability controls.
• Use a buffer containing 1-2% BSA or serum and 0.05-0.1% sodium azide for staining; avoid permeabilization unless targeting intracellular epitopes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for coagulation and hemostasis research. Whether you need rare target reagents like anti-CD142 antibodies or custom sourcing solutions, we bridge your concept to clinic needs.