Rabbit anti-Human CD142 Monoclonal Antibody for Flow Cytometry - P13726

Core Product Specifications and Parameters

Biological Background: Tissue Factor Function and Localization

• Tissue factor (TF), also known as coagulation factor III or thromboplastin, is encoded by the F3 gene and is a member of the cytokine receptor family.
• Initiates blood coagulation by forming a complex with circulating factor VII or VIIa; the [TF:VIIa] complex activates factors IX or X through specific limited proteolysis.
• Plays a central role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
• Subcellular localization includes membrane (integral transmembrane protein) and secreted forms.
• Tissue-specific expression is prominent in lung, placenta, and pancreas.
• Post-translational modifications indicated by UniProt keywords include disulfide bonds, glycosylation, and palmitoylation.
• Additional features: alternative splicing, lipoprotein association, signal peptide, and a hemostasis-related reference proteome designation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 34-251) of human CD142, suggesting the antibody likely recognizes the mature surface-exposed region.
• For flow cytometry applications, use appropriate isotype controls and include viability dyes to exclude dead cells. Validate staining in relevant human cell lines or primary cells expressing CD142.
• is a bright far-red fluorochrome; minimize light exposure and use standard APC/Cy5 filter sets. Consider titrating the antibody for optimal signal-to-noise ratio.
• Due to the transmembrane nature of TF, permeabilization is not required for surface staining, but if intracellular staining is desired, include proper permeabilization controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined, single-point solution for IVD raw-material procurement, from discovery through commercialization. In the area of hemostasis and coagulation, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can handle rare-target sourcing needs. Our portfolio includes high-quality conjugated antibodies such as this anti-CD142 (TF) product, ensuring consistent supply for your diagnostic development programs.