Rabbit anti-Human CD141/Thrombomodulin Monoclonal Antibody for Flow Cytometry - P07204

Core Product Specifications and Parameters

Biological Background: Thrombomodulin Function and Localization

• Thrombomodulin (CD141/BDCA-3, also known as fetomodulin) is a transmembrane receptor abundantly expressed on vascular endothelial cells, functioning in hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis. Related references: PMID:10761923
• It acts as a cofactor for thrombin-mediated activation of protein C on the endothelial surface, initiating the anticoagulant pathway. Related references: PMID:29323190, PMID:33836597, PMID:9395524
• Thrombomodulin accelerates activation of plasma carboxypeptidase B2 (CPB2), which cleaves C-terminal basic amino acids from kinins and anaphylatoxins, contributing to fibrinolysis inhibition. Related references: PMID:26663133
• It suppresses inflammation by sequestering the pro-inflammatory molecule HMGB1, preventing its interaction with RAGE and other receptors. Related references: PMID:15841214
• The protein alters the cleavage specificity of protease-activated receptor 1 (PAR1), thereby inhibiting endothelial permeability and inflammation (By similarity).
• Subcellular localization: predominantly membrane-bound.
• Tissue specificity: expression is unique to endothelial cells.
• Structural features include an EGF-like domain, disulfide bonds, glycosylation, and a proteoglycan modification; the mature protein migrates at approximately 60 kDa.

Experimental Guidance and Technical Tips

• This conjugate is intended for direct immunofluorescence staining and flow cytometric detection of human CD141/Thrombomodulin on the surface of live or fixed cells.
• Optimal antibody concentration should be determined by titration in the relevant experimental system; a starting range of 0.5–5 µg/mL is often used for cell surface staining.
• Use appropriate isotype control and viability dye to discriminate live cells and minimize background.
• For sample preparation, avoid over-digestion with trypsin as thrombomodulin is trypsin-sensitive; consider using non-enzymatic dissociation reagents.
• CD141 is highly expressed on vascular endothelial cells; consider using primary endothelial cell cultures or peripheral blood endothelial progenitor cells for positive control.
• Note that the antibody recognizes an extracellular epitope; permeabilization is not required for surface staining but may be employed for intracellular pools if needed after fixation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CD141/Thrombomodulin-related research and assay development, we offer validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our sourcing capabilities extend to rare targets and custom formulations to support your hemostasis and inflammation diagnostic projects.