Rabbit anti-Human CD141/Thrombomodulin Monoclonal Antibody for FC - P07204

Core Product Specifications and Parameters

Biological Background: Thrombomodulin Function and Localization

• Thrombomodulin (also known as Fetomodulin) is a transmembrane receptor encoded by the THBD gene in humans.
• It acts as a cofactor for thrombin-mediated activation of protein C on vascular endothelial cells, initiating the anticoagulant pathway. Related references: PMID:10761923 PMID:29323190 PMID:33836597 PMID:9395524
• Thrombomodulin accelerates the activation of plasma carboxypeptidase B2 (CPB2), which removes C-terminal basic amino acids from kinins and anaphylatoxins, thereby inhibiting fibrinolysis. Related references: PMID:26663133
• It plays protective roles by modifying the cleavage specificity of protease-activated receptor 1 (PAR1), reducing endothelial cell permeability and inflammation.
• Independent of its anticoagulant cofactor activity, thrombomodulin suppresses inflammation by sequestering HMGB1, preventing its engagement with pro-inflammatory receptors such as RAGE. Related references: PMID:15841214
• Subcellularly, thrombomodulin is localized to the membrane, consistent with its receptor function.
• It is uniquely synthesized in endothelial cells, highlighting its vascular-specific physiological roles.

Experimental Guidance and Technical Tips

• This conjugate enables direct fluorescent detection of human CD141/Thrombomodulin via flow cytometry without the need for a secondary antibody.
• The immunogen spans the extracellular domain (amino acids 19–515), suggesting the antibody likely recognizes an epitope accessible on the cell surface of live, unfixed cells.
• Given the endothelial-specific expression, consider using primary human endothelial cells or cell lines such as HUVEC as positive controls; validate expression in your particular experimental system.
• Factor in that thrombomodulin can be shed from the cell surface under certain conditions; include appropriate untreated controls and consider stimulation or inhibition treatments to assess surface retention.
• As cross-reactivity is limited to human, the antibody is not suitable for mouse or rat model studies unless used in human xenograft contexts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering a comprehensive range of antibodies, antigens, and auxiliary reagents tailored to diagnostic development. For researchers and manufacturers studying thrombomodulin and related coagulation/inflammation pathways, we provide access to validated antibody pairs, conjugated monoclonal antibodies, and bulk ancillary reagents, ensuring consistent supply for your assay workflows. Our specialized sourcing capabilities also extend to rare targets, helping you accelerate from concept to clinic with a single partner.