Rabbit anti-Human CD140b/PDGFRβ Monoclonal Antibody for Flow Cytometry - P09619

Core Product Specifications and Parameters

Biological Background: Platelet-derived growth factor receptor beta Function and Localization

• Protein identity: Platelet-derived growth factor receptor beta (PDGFRβ, CD140b) is a receptor tyrosine kinase that binds PDGFB, PDGFD, and PDGFA/PDGFB heterodimers, playing essential roles in cell proliferation, migration, and survival.
• Core function: Acts as a key regulator of embryonic development and cardiovascular system formation, including pericyte and smooth muscle cell recruitment to endothelial cells, and normal kidney glomerulus capillary network formation.
• Signaling cascades: Upon ligand binding, PDGFRβ activates multiple downstream pathways including PLCG1, PI3K-AKT, and HRAS-MAPK cascades, promoting actin cytoskeleton rearrangement and membrane ruffling.
• Subcellular localization: Localizes to the cell membrane, cytoplasmic vesicles, and lysosome lumen, consistent with its role in signal transduction and receptor internalization.
• Post-translational modifications and keywords: Phosphorylation, glycoprotein, disulfide bond, and ubiquitination modifications are characteristic; the protein contains immunoglobulin-like domains and a tyrosine kinase domain.
• Clinical relevance: Mutations and chromosomal rearrangements in PDGFRB are associated with various diseases including cancers, making it a proto-oncogene and therapeutic target.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 33-530) covers most of the extracellular domain, making this antibody suitable for detection of native cell surface PDGFRβ by flow cytometry.
• Consider using appropriate isotype controls and Fc receptor blocking to ensure specific staining in flow cytometry experiments.
• Since PDGFRβ is expressed on pericytes, vascular smooth muscle cells, and some mesenchymal cells, validate antibody staining in relevant cell lines or primary cells.
• For applications other than flow cytometry, test the antibody in appropriate assay conditions, as it has been validated only for FC by the supplier.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive sourcing partner for IVD raw materials, offering end-to-end support from early-stage research to clinical application. This anti-PDGFRβ antibody supports studies in cardiovascular biology, oncology, and cell signaling, areas where CamelBio provides a wide range of validated antibodies, recombinant proteins, and ancillary reagents. Whatever your diagnostic development needs, CamelBio delivers reliable, high-quality raw materials and expert technical services to accelerate your projects.