Rabbit anti-Human CD13/ANPEP Monoclonal Antibody for Flow Cytometry - P15144

Core Product Specifications and Parameters

Biological Background: Aminopeptidase N Function and Localization

• Aminopeptidase N (ANPEP), also known as CD13 or gp150, is a zinc-dependent metalloprotease belonging to the M1 family of aminopeptidases, characterized by a large extracellular catalytic domain.
• It plays a central role in the final digestion of peptides produced from gastric and pancreatic proteolysis, and processes various bioactive peptides including angiotensin III/IV, neuropeptides, and chemokines.
• ANPEP localizes to the cell membrane as a type II transmembrane protein, with a short cytoplasmic tail and signal-anchor domain, positioning its active site extracellularly.
• Expression is widespread in epithelial cells of the kidney, intestine, and respiratory tract, as well as in granulocytes, monocytes, endothelial cells, and cerebral pericytes; a soluble form is elevated in plasma and effusions of cancer patients.
• The protein serves as a functional receptor for human coronavirus 229E (HCoV-229E) and mediates human cytomegalovirus (HCMV) infection.
• Post-translational modifications include N-linked glycosylation, sulfation, disulfide bond formation, and coordination of a catalytic zinc ion essential for enzymatic activity.
• ANPEP is implicated in angiogenesis, cholesterol crystallization, and amino acid transport via interaction with SLC6A19, and is differentially expressed in tumor vasculature and malignant gliomas.
• The UniProt record classifies it with keywords including hydrolase, protease, receptor, host-virus interaction, and differentiation, underscoring its multi-functional roles in health and disease.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein spanning the extracellular region (aa 69–967), which likely presents native-like epitopes; the resulting monoclonal antibody is expected to recognize the native protein on live cells in flow cytometry.
• For FC applications, consider using human peripheral blood leukocytes, THP-1 monocytic cells, or HUVEC endothelial cells as positive controls based on reported CD13 expression.
• Include appropriate isotype controls and validate the staining pattern against known expression profiles; titrate the antibody for optimal signal-to-noise ratio.
• Confirm specificity in your experimental system, as cross-reactivity with other species or aminopeptidases may need assessment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research laboratories to provide a comprehensive portfolio of validated IVD raw materials, including high-quality monoclonal antibodies such as this anti-CD13/ANPEP clone. With expertise in bulk ancillary reagents, optimized antibody pairs, and rare target sourcing, we support your development of flow cytometry-based assays and immuno-oncology research. From concept to clinic, rely on CamelBio as your trusted supply chain bridge for critical raw-material needs.