Rabbit anti-Human CD13/ANPEP Monoclonal Antibody for Flow Cytometry - P15144

Core Product Specifications and Parameters

Biological Background: CD13/ANPEP Function and Localization

• Aminopeptidase N (CD13/ANPEP) is a broad-specificity zinc-dependent metalloprotease that plays a key role in the final digestion of peptides generated from gastric and pancreatic proteolysis.
• It processes various peptide hormones, including angiotensin III and IV, neuropeptides, and chemokines, and may cleave peptides bound to MHC class II molecules on antigen-presenting cells.
• CD13 is implicated in angiogenesis and promotes cholesterol crystallization, and may regulate amino acid transport by interacting with the transporter SLC6A19.
• As a viral receptor, CD13 mediates entry of human coronavirus 229E (HCoV-229E) and human cytomegalovirus (HCMV) into host cells.
• The protein is expressed on the cell membrane of epithelial cells in kidney, intestine, and respiratory tract, as well as on granulocytes, monocytes, endothelial cells, and cerebral pericytes.
• A soluble form of CD13 is found in plasma and is elevated in cancer patients; it is also detected in tumor vasculature and metastatic lymph nodes.
• Key structural features include disulfide bonds, N-glycosylation, and sulfation, with a molecular weight of approximately 110 kDa (mature form).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 69–967, covering the majority of the extracellular domain; this design may offer good epitope accessibility for live-cell flow cytometry staining.
• As an -conjugated monoclonal antibody, it is ready-to-use for direct immunofluorescence; always include appropriate isotype and unstained controls to confirm specificity.
• Validate the antibody in your specific human sample system (e.g., cell lines or primary cells known to express CD13) under optimal staining conditions.
• Since CD13 is a cell-surface marker, avoid permeabilization unless intracellular staining is intended; note that soluble CD13 in culture supernatant may compete for binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with reliable access to a broad portfolio of IVD raw materials, including validated antibody pairs, engineered monoclonal antibodies, and ancillary reagents. With expertise in targets like CD13/ANPEP—relevant to viral entry, cancer, and immunology—we help streamline your assay development from concept to clinic. Contact us to explore bulk supply and custom sourcing solutions for your specific workflow needs.