Rabbit anti-Human CD138/Syndecan-1 Monoclonal Antibody for Flow Cytometry - P18827

Core Product Specifications and Parameters

Biological Background: CD138/Syndecan-1 Function and Localization

• Syndecan-1 (CD138) is a cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate chains, linking the cytoskeleton to the interstitial matrix.
• It regulates exosome biogenesis in concert with SDCBP and PDCD6IP. Related references: PMID:22660413
• Syndecan-1 can induce its own expression in dental mesenchymal cells and neighboring dental epithelial cells via an MSX1-mediated pathway.
• The protein is detected in placenta and fibroblasts at the protein level, indicating tissue-specific expression patterns.
• Subcellularly, syndecan-1 localizes to the cell membrane, is secreted, and is found in extracellular exosomes.
• Post-translational modifications include glycosylation with heparan sulfate and chondroitin sulfate, as well as phosphorylation, contributing to its functional diversity.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular region (aa 23-251), which is well-suited for detecting cell surface CD138 in flow cytometry.
• For flow cytometry, consider titrating the antibody to determine the optimal concentration for your specific cell type and application.
• is a bright fluorophore excitable by the violet (~405 nm) laser; ensure your cytometer configuration is compatible.
• Validate the antibody in your relevant sample system, using appropriate isotype controls and positive/negative cell populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with comprehensive IVD raw material sourcing, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-CD138 antibody addresses flow cytometry needs for immunophenotyping, particularly in plasma cell research and exosome characterization. Leverage our one-stop supply chain to streamline your project from concept to clinic.