Rabbit anti-Human CD138 (Syndecan-1) Monoclonal Antibody for Flow Cytometry - P18827

Core Product Specifications and Parameters

Biological Background: CD138/Syndecan-1 Function and Localization

• Syndecan-1 (CD138) is a transmembrane proteoglycan that carries both heparan sulfate and chondroitin sulfate glycosaminoglycan chains, mediating cell–matrix interactions.
• It regulates exosome biogenesis through a complex with SDCBP and PDCD6IP, impacting intercellular communication. Related references: PMID:22660413
• Encoded by the SDC1 gene, it belongs to the syndecan family of cell surface proteoglycans, which link the cytoskeleton to the interstitial matrix.
• Subcellularly, Syndecan-1 is localized at the plasma membrane, can be secreted, and is a component of extracellular exosomes.
• At the protein level, it is detected in placenta and fibroblasts, reflecting a role in tissue organization and remodeling. Related references: PMID:32337544, PMID:36213313
• Key post-translational modifications include glycophosphatidylinositol (GPI) anchoring, extensive N- and O-glycosylation, and phosphorylation, contributing to its structural and functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 23–251, which covers the extracellular domain excluding the signal peptide; this design may preferentially detect membrane-bound or shed forms of CD138.
• For flow cytometry, consider titrating the antibody from 0.1–1.0 µg per million cells in a suspension of known CD138-expressing lines (e.g., plasma cells or tumor lines) to determine optimal staining.
• Always include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering a comprehensive portfolio that includes this CD138 monoclonal antibody alongside validated antibody pairs and ancillary reagents. For diagnostic developers focused on oncology or exosome-based liquid biopsies, we support bulk orders and technical consultation spanning concept to clinical stages. Our network of certified suppliers ensures consistent quality across monoclonal and polyclonal antibodies, rare target sourcing, and tailored procurement solutions.