Rabbit anti-Human CD134/OX40 mAb for FC - P43489

Core Product Specifications and Parameters

Biological Background: TNFRSF4 (CD134/OX40) Function and Localization

• Tumor necrosis factor receptor superfamily member 4 (TNFRSF4), also known as CD134, OX40, ACT35 antigen, OX40L receptor, and TAX transcriptionally-activated glycoprotein 1 receptor. It is a single-pass type I membrane protein containing a signal peptide, four cysteine-rich repeats in the extracellular domain, a transmembrane helix, and a cytoplasmic tail.
• Functions as a receptor for TNFSF4/OX40L/GP34, providing costimulatory signals that promote long-term T-cell immunity, survival, and cytokine production.
• Serves as a host cell receptor for human herpesvirus 6B (HHV-6B) entry during microbial infection.
• Subcellular localization: Membrane; specifically expressed on the surface of activated T cells, regulatory T cells, and some other immune cells.
• Key structural features: Contains disulfide bonds and glycosylation sites, characteristic of the TNF receptor family. Its 3D structure has been determined.
• Molecular weight: approximately 29.3 kDa (UniProt), consistent with the observed 29 kDa.
• The immunogen (amino acids 29-216) encompasses the extracellular domain, ensuring recognition of the native surface receptor.

Experimental Guidance and Technical Tips

• is a far-red fluorescent dye (excitation max ~650 nm, emission ~668 nm); use appropriate laser lines (e.g., 633/635 nm) and filters (e.g., 660/20) for detection.
• This monoclonal antibody is directly conjugated, eliminating the need for a secondary antibody and reducing background in flow cytometry.
• Since the immunogen targets the extracellular domain, it should bind to live, unfixed cells. For optimal staining, titrate the antibody to determine the best concentration for your sample type.
• Include appropriate isotype controls and viability dyes to set gates accurately.
• Validate antibody performance in your specific experimental system, especially if using after fixation/permeabilization, as epitopes may be altered.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with a comprehensive range of IVD raw materials. This anti-human CD134/OX40 monoclonal antibody supports flow cytometry applications in T-cell biology and immunotherapy research. Beyond this conjugate, CamelBio can source validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and rare-target raw materials to streamline your diagnostic development from concept to clinic.