Rabbit anti-Human CD131 mAb for Flow Cytometry - P32927

Core Product Specifications and Parameters

Biological Background: CD131 Function and Localization

• CD131 (CSF2RB) is a cytokine receptor common beta subunit critical for the signaling of interleukin-3, interleukin-5, and granulocyte-macrophage colony-stimulating factor (GM-CSF). It is also designated CDw131 and betaGMR.
• It functions by forming heterodimeric complexes with specific alpha subunits: IL3RA, IL5RA, or CSF2RA, mediating the production and differentiation of hematopoietic progenitor cells. Related references: PMID:1495999
• Ligand binding triggers JAK1 activation and downstream JAK-STAT pathway signaling, controlling immune cell proliferation and survival. Related references: PMID:9516124
• Subcellularly, CD131 is a single-pass transmembrane protein localized at the plasma membrane.
• Post-translational modifications include glycosylation and phosphorylation, and the protein undergoes alternative splicing, producing multiple isoforms.
• The calculated molecular weight of the unmodified protein is approximately 97 kDa, consistent with the observed 97 kDa band.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 17–443); the conjugated antibody is suitable for direct live-cell flow cytometry without a secondary detection step.
• Titrate the antibody to determine the optimal staining concentration for your specific cell type and flow cytometer settings.
• Include appropriate isotype controls and fluorescence compensation controls when performing multicolor flow cytometry panels.
• Validate staining in relevant human hematopoietic cell lines or primary immune cells known to express CD131.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to high-quality IVD raw materials and technical support. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for immune signaling and cytokine receptor research. For rare or custom targets like CD131, our sourcing team can accelerate your development from concept to clinic.