Rabbit anti-Human CD131 mAb for FC - P32927

Core Product Specifications and Parameters

Biological Background: CD131 Function and Localization

• Cytokine receptor common subunit beta (CD131, CSF2RB) is a 97 kDa type I transmembrane glycoprotein encoded by the CSF2RB gene, which also yields alternative splicing isoforms. It is a member of the type I cytokine receptor family and serves as the shared signal-transducing subunit for the interleukin-3 (IL-3), interleukin-5 (IL-5), and granulocyte-macrophage colony-stimulating factor (GM-CSF) receptors.
• CD131 forms high-affinity heterodimeric receptors by pairing with ligand-specific alpha subunits (IL3RA, IL5RA, or CSF2RA). Ligand binding triggers receptor activation and downstream signaling. Related references: PMID:1495999
• In unstimulated cells, CD131 constitutively associates with JAK1. Upon ligand engagement, JAK1 is activated, leading to phosphorylation and stimulation of the JAK-STAT pathway, which regulates gene expression and cell proliferation. Related references: PMID:9516124
• CD131 is primarily localized to the plasma membrane, consistent with its role as a cell surface receptor. It contains an extracellular ligand-binding domain, a single transmembrane helix, and a cytoplasmic tail involved in signal transduction.
• The protein undergoes co- and post-translational modifications, including N-linked glycosylation and phosphorylation, which are critical for its stability, surface expression, and signaling function.
• CD131 plays a central role in hematopoietic cell survival, proliferation, and differentiation, and is essential for normal immune responses and inflammatory processes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 17–443) of human CD131, suggesting recognition of the native, membrane-proximal region. This makes the antibody suitable for detecting surface-expressed CD131 without permeabilization.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type. Include appropriate isotype controls and Fc receptor blocking steps to minimize non-specific binding.
• CD131 is expressed on a variety of hematopoietic cells, including monocytes, eosinophils, basophils, and hematopoietic progenitor cells. Validate the antibody in your target cell population, such as peripheral blood mononuclear cells (PBMCs) or relevant cell lines.
• As a conjugated antibody, avoid repeated freeze-thaw cycles and protect from prolonged light exposure to preserve fluorochrome stability and antibody activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research facilities with streamlined access to a comprehensive portfolio of IVD raw materials, technical expertise, and consultation services. For targets like CD131 in cytokine signaling and hematopoiesis research, we offer validated antibody pairs, optimized monoclonal antibodies (including conjugated formats), and bulk ancillary reagents. Our end-to-end supply chain and rare-target sourcing capabilities help you advance from concept to clinical application efficiently.