Rabbit anti-Human CD120b/TNFRSF1B mAb for FC - P20333

Core Product Specifications and Parameters

Biological Background: TNFRSF1B Function and Localization

• TNFRSF1B (also known as TNFR2, p75, CD120b) is a member of the tumor necrosis factor receptor superfamily. It is a high-affinity receptor for TNF and has lower affinity for lymphotoxin-alpha.
• The receptor mediates most of the metabolic effects of TNF and plays a role in apoptosis regulation through the recruitment of TRAF1/TRAF2 complex, which then recruits apoptotic suppressors BIRC2 and BIRC3.
• Alternatively spliced isoform 2 lacking the transmembrane domain is secreted and blocks TNF-induced apoptosis, suggesting a regulatory function by antagonizing TNF biological activity.
• Localized primarily to the cell membrane as a single-pass type I membrane protein; a secreted form exists due to alternative splicing or proteolytic cleavage.
• Contains disulfide bonds and is extensively glycosylated, contributing to observed protein weights (e.g., ~48 kDa predicted, with additional bands due to modifications). It is also phosphorylated.
• The protein has implications in pharmaceutical research, being a target for anti-inflammatory therapies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (23-257), suggesting the antibody targets the extracellular region, making it suitable for live-cell flow cytometry without permeabilization.
• For best results in flow cytometry, titrate the antibody to determine the optimal concentration for your specific cell type and experimental conditions.
• Include appropriate isotype controls and viability dyes to distinguish specific staining from background and dead cells.
• Given the potential shedding of TNFR2, consider analyzing both surface and soluble receptor forms if studying regulation.
• Since the antibody is directly conjugated to , it enables one-step staining, reducing assay time and potential variability from secondary reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a streamlined supply chain for IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and ancillary reagents. This TNFRSF1B/TNFR2 antibody supports apoptosis and inflammatory disease research, and CamelBio can source additional rare targets or bulk reagents upon request.