Rabbit anti-Human CD11a Monoclonal Antibody for FC - P20701

Core Product Specifications and Parameters

Biological Background: Integrin alpha-L Function and Localization

• Integrin alpha-L (ITGAL, CD11a) pairs with integrin beta-2 (ITGB2) to form the leukocyte function-associated molecule-1 (LFA-1) receptor, which binds intercellular adhesion molecules ICAM1, ICAM2, ICAM3, and ICAM4. Related references: PMID:10846180, PMID:12526797, PMID:1676048
• LFA-1 also functions as a receptor for F11R (junctional adhesion molecule A) and the secreted ubiquitin-like protein ISG15, with the latter interaction mediated specifically by ITGAL. Related references: PMID:11812992, PMID:15528364, PMID:29100055
• ITGAL is critical for leukocyte-endothelial cell interactions, cytotoxic T-cell mediated killing, antibody-dependent cellular cytotoxicity by granulocytes and monocytes, and natural killer cell cytotoxicity. Related references: PMID:15356110
• The protein mediates leukocyte adhesion and transmigration, including T-cell and neutrophil trafficking, and contributes to the immunological synapse by translating TCR engagement and ICAM1 density into graded adhesion. Related references: PMID:11812992, PMID:38195629
• In association with ICAM3, ITGAL facilitates phagocytosis of apoptotic neutrophils by macrophages. Related references: PMID:23775590
• As a receptor for the neuron-specific adhesion molecule ICAM5, it ensures neuron-leukocyte adhesion. Related references: PMID:10741396
• ITGAL is required for the generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis.
• Subcellularly, ITGAL localizes to the cell membrane and membrane rafts, and is expressed specifically on leukocytes. Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation; it also undergoes alternative splicing. The protein binds calcium and magnesium and is involved in host-virus interactions.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein corresponding to the extracellular domain (aa 26-340) of human CD11a; consider its suitability for detecting surface-expressed antigen under non-denaturing conditions.
• This conjugate is validated for flow cytometry; for optimal staining, titrate the antibody to determine the appropriate working concentration in your cell system.
• Use appropriate isotype controls and include viability dyes to discriminate live leukocyte populations.
• While not validated for other applications, the clone may be compatible with immunofluorescence or functional assays; pilot testing with relevant controls is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This CD11a antibody supports immunology-focused assay development targeting LFA-1-mediated adhesion and immune cell function. Our sourcing capabilities span rare target raw materials, helping you accelerate projects from concept to clinic.