Rabbit anti-Human CD11a mAb - P20701

Core Product Specifications and Parameters

Biological Background: Integrin alpha-L Function and Localization

• Integrin alpha-L (ITGAL/CD11a) pairs with integrin beta-2 (ITGB2) to form the leukocyte function-associated antigen-1 (LFA-1) complex, a key adhesion receptor on leukocytes.
• LFA-1 binds to intercellular adhesion molecules ICAM1, ICAM2, ICAM3, and ICAM4, mediating leukocyte-endothelial cell interactions and T-cell migration. Related references: PMID:10846180 PMID:12526797 PMID:1676048
• It also acts as a receptor for F11R and the ubiquitin-like protein ISG15, expanding its role in immune regulation. Related references: PMID:11812992 PMID:15528364 PMID:29100055
• Integrin alpha-L is essential for cytotoxic T-cell-mediated killing, antibody-dependent cellular cytotoxicity, and natural killer cell cytotoxicity. Related references: PMID:15356110
• The protein localizes to the cell membrane and membrane rafts, positioning it for dynamic adhesion during immune synapse formation.
• Post-translational modifications include phosphorylation, and the protein contains disulfide bonds, calcium-binding sites, and transmembrane domains, reflecting its integrin family membership.
• Notably, alternative splicing generates multiple isoforms, and the protein is involved in phagocytosis of apoptotic neutrophils. Related references: PMID:23775590
• Tissue expression is restricted to leukocytes, consistent with its immune-specific functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (aa 26–340), which contains ligand-binding domains; consider this for potential blocking or conformational effects.
• For flow cytometry, titrate the antibody to determine the optimal concentration for human leukocyte samples; include appropriate isotype controls.
• Validate the antibody in the relevant sample system (e.g., human PBMCs) using standard FC protocols; note that cross-reactivity is reported only for human.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, including validated antibody pairs, monoclonal antibodies, and ancillary reagents. For researchers studying leukocyte adhesion and immune cell function, we supply high-quality antibodies like anti-CD11a to accelerate your assay development. Our supply capabilities span bulk reagents and rare target sourcing, supporting your journey from concept to clinic.