Rabbit anti-Human CD11a mAb for FC - P20701

Core Product Specifications and Parameters

Biological Background: CD11a (Integrin alpha-L) Function and Localization

• CD11a, also known as integrin alpha-L or LFA-1 alpha chain, is encoded by the ITGAL gene and serves as a leukocyte-specific adhesion molecule.
• Paired with integrin beta-2 (ITGB2), it forms the LFA-1 heterodimer, which acts as a receptor for intercellular adhesion molecules (ICAM1, ICAM2, ICAM3, ICAM4) to mediate leukocyte adhesion and immune synapse formation. Related references: PMID:10846180 PMID:12526797 PMID:1676048
• LFA-1 engagement triggers downstream signaling critical for leukocyte-endothelial transmigration, T-cell cytotoxicity, and natural killer cell function. Related references: PMID:11812992 PMID:15356110
• It also acts as a receptor for non-classical ligands, including F11R, secreted ISG15, and neuronal ICAM5, expanding its role beyond classical immune adhesion. Related references: PMID:15528364 PMID:29100055 PMID:10741396
• Subcellularly, CD11a localizes primarily to the plasma membrane and enriches in membrane rafts upon activation.
• Tissue expression is mainly restricted to leukocytes, consistent with its immune-restricted functions.
• The protein undergoes N-glycosylation and alternative splicing, resulting in observed molecular weight variants (118/129/134 kDa) depending on glycosylation state.
• Key biological keywords include calcium/magnesium-dependent adhesion, host-virus interaction, phagocytosis, and phosphoprotein signaling.

Experimental Guidance and Technical Tips

• This -conjugated antibody is intended for flow cytometry on human leukocyte samples. To determine optimal staining concentration, perform a titration series in your assay system.
• Given widespread leukocyte expression of CD11a, consider using appropriate isotype controls and Fc receptor blocking to reduce non-specific binding.
• The immunogen (aa 26-340) spans the extracellular ligand-binding domain, suggesting suitability for detecting native surface CD11a; however, fixation and permeabilization may alter epitope availability.
• When co-staining with other leukocyte markers, verify fluorochrome compatibility and include single-color compensation controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with streamlined access to validated IVD raw materials, including the anti-CD11a monoclonal antibody for flow cytometry. Beyond individual reagents, our portfolio encompasses optimized antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents. For projects requiring rare or custom targets, CamelBio offers tailored sourcing solutions to accelerate your development from concept to clinic.