Rabbit anti-Human CD119/IFNGR1 Monoclonal Antibody for Flow Cytometry - P15260

Core Product Specifications and Parameters

Biological Background: CD119/IFNGR1 Function and Localization

• Interferon gamma receptor 1 (IFNGR1, CD119) is a cell surface receptor subunit that mediates crucial immune responses against pathogens and tumors.
• Upon ligand binding, IFNGR1 forms a functional receptor complex with accessory factor IFNGR2, triggering intracellular signaling. Related references: PMID:10986460 PMID:2971451 PMID:7615558
• The intracellular domain of IFNGR1 undergoes conformational changes that allow association with JAK1 and JAK2 kinases; activated JAK1 phosphorylates IFNGR1 to create a docking site for STAT1. Phosphorylated STAT1 dimerizes, translocates to the nucleus, and stimulates transcription of interferon-responsive genes. STAT3 is also weakly activated. Related references: PMID:28883123
• Phosphorylated IFNGR1 also provides a binding site for SOCS1, which competes with STAT1 to negatively regulate the JAK-STAT signaling cascade.
• Through this pathway, IFNGR1 activates effector immune cells and enhances antigen presentation, underpinning antimicrobial, antiviral, and antitumor responses. Related references: PMID:20015550
• IFNGR1 is localized to the cell membrane.
• The protein is a glycoprotein containing disulfide bonds and an immunoglobulin-like domain. Alternative splicing generates isoforms, some of which may be secreted or have distinct functions.
• Mutations in IFNGR1 are linked to primary immunodeficiencies, including autosomal recessive (IMD27A) and autosomal dominant (IMD27B) forms, characterized by increased susceptibility to mycobacterial infections.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 18-245) encompasses the extracellular domain of human IFNGR1; thus, this -conjugated antibody is designed for detection of surface CD119 by flow cytometry.
• To achieve specific staining, titrate the antibody on relevant cell lines or primary samples, and include appropriate isotype and fluorescence-minus-one (FMO) controls.
• CD119 is expressed on diverse immune cell types (e.g., T and B lymphocytes, macrophages, dendritic cells). Consider co-staining with lineage markers to dissect cell subsets.
• As the antibody targets the extracellular region, it may interfere with IFN-gamma binding in functional assays; this possibility should be experimentally validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined, single-vendor model for IVD raw-material sourcing. From this anti-CD119 monoclonal antibody for flow cytometry to complementary assay components such as recombinant IFN-gamma receptor proteins, detection antibodies, and bulk ancillary reagents, CamelBio provides tailored procurement solutions. Wherever your work focuses—immune monitoring, cytokine signaling, or primary immunodeficiency studies—CamelBio is committed to bridging your innovation from concept to clinic.