Rabbit anti-Human CD119/IFNGR1 Monoclonal Antibody for Flow Cytometry (FC) - P15260

Core Product Specifications and Parameters

Biological Background: CD119/IFNGR1 Function and Localization

• Interferon gamma receptor 1 (IFNGR1), also known as CD119 or IFN-gamma-R-alpha, is the ligand-binding subunit of the interferon gamma receptor.
• It plays crucial roles in antimicrobial, antiviral, and antitumor immune responses by activating effector immune cells and enhancing antigen presentation (PubMed:20015550). Related references: PMID:20015550
• Upon associating with the accessory factor IFNGR2, the functional receptor binds interferon gamma with high affinity, triggering downstream signaling (PubMed:10986460, PubMed:2971451, PubMed:7615558). Related references: PMID:10986460 PMID:2971451 PMID:7615558
• Ligand binding leads to phosphorylation and activation of JAK1 and JAK2, which in turn phosphorylate IFNGR1 to create a docking site for STAT1; activated STAT1 dimerizes and translocates to the nucleus to regulate target gene transcription (PubMed:28883123). Related references: PMID:28883123
• The intracellular domain also recruits SOCS1 via phosphorylation, which negatively regulates JAK-STAT signaling (By similarity).
• Subcellularly, IFNGR1 is a single-pass type I membrane protein localized to the cell membrane.
• Post-translational modifications include N-glycosylation and phosphorylation; it contains an extracellular immunoglobulin-like domain and forms disulfide bonds.
• Keywords from UniProt: alternative splicing, disease variant, reference proteome, transmembrane helix, and Ubl conjugation, reflecting its structural features and involvement in human pathologies.

Experimental Guidance and Technical Tips

• The recombinant protein immunogen suggests that the antibody likely recognizes either linear or conformational epitopes of human CD119/IFNGR1; validate specificity in the appropriate sample system.
• The fluorescent conjugate enables direct detection by flow cytometry; optimize antibody titration and use appropriate isotype controls for FC experiments.
• Ensure proper compensation settings when combining with other fluorophores, and consider viability dyes to exclude dead cells.
• For staining, follow standard protocols for surface antigen detection on live cells; fixation may be applied after staining if needed.
• The antibody is intended for research and IVD raw material applications; end-users should perform necessary validation and quality control for their specific assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, supplying this CD119/IFNGR1 antibody along with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. We support diagnostic manufacturers and research institutes from concept to clinic, with particular expertise in immune-response and infectious disease targets. Contact us for additional formats, bulk supply, and rare target raw-material solutions tailored to your assay development needs.