Rabbit anti-Human CD117/c-Kit Monoclonal Antibody for Flow Cytometry - P10721

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• KIT is a receptor tyrosine kinase that binds stem cell factor (KITLG/SCF) and regulates cell survival, proliferation, and differentiation in hematopoiesis, gametogenesis, melanogenesis, and mast cell biology.
• Upon ligand binding, KIT activates multiple downstream signaling cascades, including PI3K-AKT, RAS-MAPK, and STAT pathways.
• Also known as PBT, SCFR, C-Kit, CD117, and MASTC.
• The protein contains immunoglobulin-like domains, a transmembrane region, and a cytoplasmic tyrosine kinase domain.
• Subcellular location is primarily the cell membrane, with potential cytoplasmic localization.
• In human testis, KIT is expressed in spermatogonia and Leydig cells, and is maintained in ejaculated spermatozoa. Related references: PMID:20601678
• Alternative splicing generates multiple isoforms; the canonical sequence is 976 amino acids with a molecular weight of ~110 kDa.
• KIT is a proto-oncogene, and gain-of-function mutations are associated with several cancers, including gastrointestinal stromal tumors and mastocytosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (aa 26-520), suggesting that the antibody recognizes the ligand-binding region; confirm with appropriate controls.
• The conjugate is designed for flow cytometry; titrate the antibody in the relevant cell type to determine the optimal staining concentration.
• Validate reactivity in target cell populations such as hematopoietic stem cells, mast cells, or kit-expressing cell lines.
• Consider using appropriate isotype controls and viability dyes for accurate gating.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material partner, CamelBio connects diagnostic manufacturers and researchers to high-quality reagents like this anti-CD117/c-Kit monoclonal antibody, supporting immuno-oncology and hematology applications. We offer validated antibody pairs, optimized monoclonal antibodies, and ancillary reagents for assay development. For rare or bulk raw-material needs, CamelBio facilitates sourcing to streamline your pipeline from discovery to clinical application.