Rabbit anti-Human CD117/c-Kit mAb for Flow Cytometry - P10721

Core Product Specifications and Parameters

Biological Background: CD117/c-Kit Function and Localization

• CD117/c-Kit is a receptor tyrosine kinase encoded by the KIT gene, also known as mast/stem cell growth factor receptor, SCFR, or Piebald trait protein (PBT).
• It serves as the cell-surface receptor for the cytokine KITLG/SCF, playing essential roles in cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis.
• Upon ligand binding, c-Kit activates multiple downstream signaling cascades, including PI3K-AKT, RAS-MAPK, and JAK-STAT pathways, through phosphorylation of adaptors like PIK3R1, GRB2, and STAT proteins.
• The protein localizes to the cell membrane and can also be found in the cytoplasm, consistent with its role as a transmembrane receptor that undergoes internalization.
• In human testis, CD117 is expressed in spermatogonia and interstitial Leydig cells, and is maintained in ejaculated spermatozoa, suggesting a role in spermatogenesis. Related references: PMID:20601678
• c-Kit is classified as a proto-oncogene, and mutations or aberrant activation are associated with various cancers; it belongs to the type III receptor tyrosine kinase family and contains immunoglobulin-like domains in its extracellular region.
• Post-translational modifications include glycosylation, disulfide bond formation, and ubiquitination, which regulate its stability and signaling.
• The full-length human c-Kit protein has a predicted molecular weight of approximately 110 kDa, in agreement with the observed weight in the product specifications.

Experimental Guidance and Technical Tips

• As this antibody is designed for flow cytometry (FC), it is suitable for surface staining of live cells, targeting the extracellular domain (aa 26-520) of human CD117.
• For optimal performance, titrate the antibody to determine the appropriate concentration for your specific cell type and experimental conditions.
• When analyzing hematopoietic stem cells or mast cells, consider co-staining with lineage markers to gate CD117-positive populations.
• Given the receptor’s internalization upon ligand binding, avoid prolonged incubation with KITLG/SCF during experimental setups that require surface staining.
• Although validated for human, consider potential cross-reactivity with other species based on sequence homology; if needed, verify using the appropriate model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated monoclonal antibodies like this anti-CD117/c-Kit mAb, ideal for flow cytometry-based diagnostic applications. From optimized antibody pairs and bulk recombinant proteins to ancillary reagents, CamelBio streamlines your sourcing workflow, supporting rare target procurement and accelerating development timelines. Partner with CamelBio to bridge the gap between concept and clinic with reliable, research-use materials.