Rabbit anti-Human CD116 Monoclonal Antibody for FC - P15509

Core Product Specifications and Parameters

Biological Background: CD116 Function and Localization

• Also known as CDw116, CD116 is the alpha subunit of the receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF), a cytokine that controls the production and function of granulocytes and macrophages.
• It functions as a low-affinity receptor that, upon ligand binding, associates with the beta subunit (CSF2RB) to form a high-affinity receptor complex, initiating signal transduction.
• Signaling through CD116 promotes proliferation, differentiation, and functional activation of hematopoietic progenitor cells and mature myeloid lineage cells.
• The receptor is expressed on the surface of various hematopoietic cells, including monocytes, macrophages, neutrophils, eosinophils, and dendritic cells.
• Alternative splicing generates multiple isoforms, including secreted forms that may modulate GM-CSF bioavailability.
• Post-translational modifications include glycosylation and disulfide bond formation, which are critical for receptor folding and function.
• Mutations in CSF2RA are associated with pulmonary alveolar proteinosis, a rare lung disorder.

Experimental Guidance and Technical Tips

• The immunogen used was the extracellular domain of human CD116, which may favor detection of native surface receptor in flow cytometry.
• For flow cytometry, consider titrating the antibody to determine optimal staining concentrations, and include appropriate isotype controls and Fc receptor blocking steps.
• The conjugate provides a bright signal, making it suitable for multicolor flow cytometry panels; validate the antibody in your specific cell system, as expression levels can vary across cell types and activation states.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with high-quality IVD raw materials, including thoroughly characterized monoclonal antibodies like this anti-CD116 clone. We offer comprehensive sourcing support covering validated antibody pairs, bulk ancillary reagents, and access to rare targets—helping streamline your assay development projects in the hematology and cytokine receptor research area.