Rabbit anti-Human CD116 Monoclonal Antibody for FC - P15509

Core Product Specifications and Parameters

Biological Background: CD116 (GM-CSF Receptor Alpha) Function and Localization

• CD116, also known as granulocyte-macrophage colony-stimulating factor receptor subunit alpha (GM-CSF-R-alpha), is encoded by the CSF2RA gene and exists in multiple isoforms due to alternative splicing.
• It functions as a low-affinity receptor for granulocyte-macrophage colony-stimulating factor (GM-CSF), transducing signals that regulate the proliferation, differentiation, and activation of hematopoietic cells.
• CD116 belongs to the type I cytokine receptor family and forms a heterodimer with the common beta chain (CD131) for high-affinity GM-CSF binding.
• The receptor is primarily localized on the cell membrane but can also be found as secreted isoforms, consistent with its role in extracellular cytokine sensing.
• Key post-translational modifications include N-glycosylation and disulfide bond formation, which are important for its structural integrity and ligand interaction.
• As indicated by UniProt keywords, CD116 is a transmembrane glycoprotein receptor with implications in cell signaling, disease variants, and proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (amino acids 23-320), which is expected to recognize the native extracellular region; consider this when designing assays that require receptor binding detection.
• This antibody is provided as an conjugate, suitable for direct flow cytometry (FC) applications. Optimize titration and staining protocols in the relevant sample system.
• For FC, ensure appropriate blocking and viability staining to minimize background; single-cell suspensions from primary human hematopoietic tissues (e.g., blood, bone marrow) are typical test samples.
• Cross-reactivity is confirmed for human; reactivity with other species or isoforms should be independently validated if intended for use.
• Because CD116 is a low-affinity receptor, detection sensitivity in FC might be influenced by receptor expression levels; consider using biological controls such as GM-CSF-stimulated cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to a comprehensive portfolio of IVD raw materials. For targets such as CD116, involved in hematopoietic research and cytokine signaling, our sourcing network covers validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare-target custom procurement. Partner with us to bridge the gap from concept to clinic with reliable, traceable raw material solutions.