Rabbit anti-Human CD112/Nectin-2 Monoclonal Antibody for Flow Cytometry - Q92692

Core Product Specifications and Parameters

Biological Background: CD112/Nectin-2 Function and Localization

• Nectin-2, also known as CD112, herpes virus entry mediator B (HveB), poliovirus receptor-related protein 2 (PVRL2), is a member of the nectin family of immunoglobulin-like cell adhesion molecules. It is encoded by the NECTIN2 gene (Gene ID: 5819) and plays roles in cell adhesion and immune modulation.
• Modulator of T-cell signaling: Nectin-2 can act as either a costimulator or a coinhibitor of T-cell function, depending on the binding receptor. Interaction with CD226 stimulates T-cell proliferation and the production of cytokines including IL-2, IL-5, IL-10, IL-13, and IFN-γ; binding to PVRIG inhibits T-cell proliferation. These competitive interactions fine-tune immune responses. Related references: PMID:26755705
• Cell adhesion: Nectin-2 is a probable cell adhesion protein, facilitating homophilic and heterophilic cell-cell interactions. Related references: PMID:9657005
• Viral entry receptor: Nectin-2 serves as a host cell receptor for herpes simplex virus 1 (HSV-1) mutant Rid1, HSV-2, and pseudorabies virus (PRV), mediating viral entry.
• Subcellular localization: Localized to the cell membrane.
• Tissue specificity: Ubiquitously expressed across human tissues.
• Post-translational modifications: Classified as a glycoprotein with disulfide bonds and phosphorylation sites; alternative splicing generates multiple isoforms. The predicted molecular weight is approximately 57.7 kDa, with observed bands at 51 kDa and 57 kDa likely reflecting differential glycosylation or isoform usage.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 32–158 within the extracellular domain of human CD112/Nectin-2 (NP_001036189.1). This region likely encompasses the first immunoglobulin-like domain, important for ligand and viral interactions. Consider potential epitope masking in cell adhesion or viral binding contexts.
• This -conjugated monoclonal antibody is validated for flow cytometry (FC). For optimal performance, titrate the antibody to determine the appropriate working dilution for your specific cell system. Include appropriate isotype controls, viability dyes, and Fc blocking reagents to minimize non-specific binding.
• As cross-reactivity is only confirmed for human samples, validate performance in any alternative species or ex vivo tissues of interest.
• Due to the ubiquitous expression of Nectin-2, expression levels may vary among different cell types; verify expression patterns in your target cell population using a reference stain or additional markers if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with comprehensive IVD raw material sourcing, from early-stage development to commercialization. This Rabbit anti-Human CD112/Nectin-2 monoclonal antibody addresses demands in immuno-oncology, infectious disease, and immune profiling. Beyond this product, we offer validated antibody pairs, recombinant proteins, bulk ancillary reagents, and custom sourcing for rare or niche targets. Partner with us to streamline your raw material supply chain with a single, reliable contact.