Rabbit anti-Human CD112/Nectin-2 Monoclonal Antibody for Flow Cytometry - Q92692

Core Product Specifications and Parameters

Biological Background: Nectin-2 Function and Localization

• Nectin-2, also known as poliovirus receptor-related protein 2 (PRR2) and herpes virus entry mediator B (HveB), belongs to the nectin family and undergoes alternative splicing to generate multiple isoforms.
• It functions as a modulator of T-cell signaling, acting as a costimulatory molecule upon binding to CD226 (DNAM-1) to enhance T-cell proliferation and cytokine production (e.g., IL-2, IL-5, IL-10, IL-13, IFN-γ), or as a coinhibitory molecule when interacting with PVRIG. Related references: PMID:26755705
• Nectin-2 mediates cell-cell adhesion through homophilic and heterophilic interactions, contributing to tissue organization. Related references: PMID:9657005
• It serves as a cellular receptor for herpes simplex virus 1 (HSV-1) mutant Rid1, HSV-2, and pseudorabies virus (PRV), facilitating viral entry.
• The protein is localized to the cell membrane and is ubiquitously expressed across tissues.
• Post-translational modifications include N-linked glycosylation, phosphorylation, and disulfide bond formation; it contains Ig-like domains, a signal peptide, and a transmembrane region.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 32–158), suggesting the antibody binds an exposed epitope on live cells, making it suitable for surface staining in flow cytometry.
• As a direct conjugate, no secondary detection reagent is needed; titrate the antibody to determine the optimal concentration for your sample type to minimize background.
• Use an appropriate isotype control to distinguish specific from non-specific binding, and validate staining in the relevant cell lines or primary cells known to express CD112.
• For cell-based assays (e.g., T-cell activation or viral entry studies), consider co-staining with additional markers to characterize Nectin-2+ cell populations or functional responses.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to validated IVD raw materials, including optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. This anti-CD112/Nectin-2 conjugate is ideally suited for flow cytometric analysis of T-cell biology, immuno-oncology, and viral pathogenesis research. For integrated procurement of antibody pairs, custom conjugation, or ancillary reagents, CamelBio streamlines your supply chain from concept to clinic.