Rabbit anti-Human CD112/Nectin-2 mAb - Q92692

Core Product Specifications and Parameters

Biological Background: CD112/Nectin-2 Function and Localization

• Nectin-2 (also known as CD112, HVEB, PRR2, PVRL2) is a member of the nectin family of immunoglobulin-like cell adhesion molecules.
• It functions as a modulator of T-cell signaling, acting as either a costimulator or coinhibitor depending on its binding partner: interaction with CD226 stimulates T-cell proliferation and cytokine production (IL2, IL5, IL10, IL13, IFNG), while binding to PVRIG inhibits T-cell proliferation. Related references: PMID:26755705
• Acts as a cell adhesion protein, mediating cell-cell contacts. Related references: PMID:9657005
• Serves as a host cell receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 2 (HHV-2), and pseudorabies virus (PRV), facilitating viral entry.
• Predominantly localizes to the cell membrane.
• Subject to alternative splicing, glycosylation, phosphorylation, and disulfide bond formation, as indicated by UniProt keywords.
• Ubiquitously expressed across human tissues.

Experimental Guidance and Technical Tips

• This antibody is pre-conjugated with for direct flow cytometry; ensure instrument compatibility with the 594 nm laser line and perform appropriate compensation.
• Titrate the antibody and include relevant isotype controls to determine optimal staining concentration for your specific sample type.
• The immunogen spans extracellular residues 32-158; the antibody is expected to recognize native surface-expressed Nectin-2, but confirm cell surface staining with viability dyes or fixation protocols.
• Although validated for FC, cross-reactivity with closely related nectins (e.g., Nectin-1, Nectin-3) may occur; verify specificity in your experimental context.
• The reported molecular weights (51 kDa and 57 kDa) likely reflect glycosylation heterogeneity; take this into account if electrophoretic analysis is planned.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and research laboratories streamlined access to IVD raw materials, from monoclonal antibodies to recombinant proteins and technical support. This -conjugated anti-CD112/Nectin-2 antibody supports immuno-oncology and virology flow cytometry applications. For bulk procurement, custom conjugation, or rare-target sourcing, our supply chain covers your project from concept to clinic.