Rabbit anti-Human CD105/Endoglin mAb for FC - P17813

Core Product Specifications and Parameters

Biological Background: CD105/Endoglin Function and Localization

• Endoglin (CD105) is a homodimeric transmembrane glycoprotein and a crucial component of the TGF-β receptor complex, primarily expressed on vascular endothelial cells.
• It is essential for angiogenesis, required for normal adult vasculature integrity, and regulates vascular endothelial cell migration (PubMed:21737454, PubMed:23300529, PubMed:7894484, PubMed:17540773). Related references: PMID:21737454 PMID:23300529 PMID:7894484 PMID:17540773
• Endoglin acts as a co-receptor for TGF-β and BMP9/GDF2 signaling, mediating activation of SMAD1/5/8 and SMAD3 transcription factors (PubMed:21737454, PubMed:22347366, PubMed:23300529). Related references: PMID:21737454 PMID:22347366 PMID:23300529
• It may mediate endothelial cell adhesion through binding to integrins and RGD-containing extracellular matrix proteins (PubMed:1692830). Related references: PMID:1692830
• Subcellular localization: Endoglin is a type I transmembrane protein found on the cell surface and within endosomal compartments following internalization.
• Tissue specificity: Highly expressed on proliferating endothelial cells, with detection in umbilical vein endothelium (PubMed:10625079) and syncytiotrophoblasts of term placenta (PubMed:1692830). Related references: PMID:10625079 PMID:1692830
• The mature protein has a predicted molecular weight of ~70.6 kDa, with observed bands at 67 kDa and 70 kDa due to differential glycosylation and potential alternative splicing.
• Mutations in the ENG gene are linked to hereditary hemorrhagic telangiectasia type 1 (HHT1), an autosomal dominant vascular disorder.

Experimental Guidance and Technical Tips

• The immunogen encompasses the extracellular domain (residues 26–337), ensuring recognition of native membrane-bound CD105. Direct conjugation to permits single-step flow cytometry without secondary antibodies.
• For FC analysis, titrate the antibody (e.g., 0.5–5 µg per million cells) and include appropriate isotype controls. Use freshly isolated or fixed endothelial cells, or known CD105-expressing cell lines (e.g., HUVECs, EAHy926).
• Consider co-staining with endothelial markers (CD31, CD34) to verify specificity. Signal may vary with activation status; validate in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to verified IVD raw materials, including this validated anti-CD105 monoclonal antibody. As a dedicated one-stop sourcing partner, we supply optimized antibody pairs, recombinant proteins, ancillary reagents, and provide tailored technical consulting to support your angiogenesis or endothelial cell research and diagnostic development from concept to clinic. Let CamelBio simplify your supply chain for rare and routine targets alike.